Crispr Hek293 Cells
Crispr Hek293 Cells - Related Videos
02:55
Genetically Modifying CAR T Cells Using a CRISPR-Cas9 System
0 Views •
2025
The video outlines a process for creating genetically modified CAR T cells through the CRISPR-Cas9 System. Infecting T cells with CRISPR and CAR lentiviruses results in modifications to the target gene and the synthesis of a chimeric antigen receptor or CAR, ultimately leading to the formation of genetically modified CAR T cells.
02:53
CRISPR and crRNAs
0 Views •
2020
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea. The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
04:22
A Technique for Gene Editing in Natural Killer Cells Using CRISPR Cas9
0 Views •
2025
This video demonstrates a technique for Cas9 ribonucleoprotein-mediated genetic modification of primary natural killer (NK) cells. A Cas9 ribonucleoprotein, consisting of a Cas9 endonuclease bound to a guide RNA (gRNA) formed by base pairing a CRISPR RNA (crRNA) and a trans-activating crRNA (tracrRNA), is introduced into primary natural killer cells via electroporation. The ribonucleoprotein targets and cleaves the host DNA at the target site, leading to gene knockout via modification of the...
05:12
All-in-One CRISPR Genome Editing: A Method for Homology Directed Repair-Based Gene Knock-In in Cultured Cells Using CRISPR-Cas9 System
0 Views •
2025
In this video, we demonstrate all-in-one CRISPR-Cas9 based genome editing in cultured cells where Cas9 and sgRNA are provided as a single plasmid construct to the cells. The CRISPR-Cas9 system and desired gene to be inserted was introduced in cells through electroporation technique to facilitate successful gene editing.
08:17
Production of High-Yield Adeno Associated Vector Batches Using HEK293 Suspension Cells
0 Views •
Cited by 3 •
2024
Here, a suspension HEK293 cell-based AAV production protocol is presented, resulting in reduced time and labor needed for vector production using components that are available for research purposes from commercial vendors.
FAQs
Targeting depends on sequence matching: the guide RNA identifies a corresponding DNA sequence, and Cas9 acts at that selected site to create a break. This pairing gives the experiment a defined genomic focus rather than changing DNA indiscriminately. In HEK293 cells, the targeted break initiates repair that produces the intended genetic modification.
After Cas9 cuts, the cell’s repair route strongly influences the result. Nonhomologous end joining repairs the break through a pathway associated with knockout generation, whereas homology-directed repair supports incorporation of a planned sequence for knock-in experiments. Thus, the same targeting strategy can produce different outcomes depending on how the break is repaired.
Different editing outcomes answer different experimental questions. Researchers may generate a knockout, a knock-in, or another sequence modification in HEK293 cells, then examine how the altered cells behave. This design connects a targeted genetic change with gene function, molecular pathways, or cellular responses while keeping the investigation within a controlled biological experiment.
HEK293 cells provide a laboratory context for examining gene function and producing recombinant proteins. Applying genome editing in this established cell line allows the same experimental system to carry a targeted genetic change and support biological measurements. That combination makes the cells relevant to both mechanistic studies and protein-production research.
A basic experiment begins by selecting a gene or sequence for modification and using a guide RNA to direct a Cas nuclease to its matching DNA site. The nuclease creates the targeted break, after which the cell repairs it through nonhomologous end joining or homology-directed repair. The repair outcome determines the resulting genetic modification.
These edited cells can model disease-associated genes, test molecular pathways, and evaluate cellular responses. They can also support studies of recombinant protein production because HEK293 cells are widely used for that purpose. The value of the approach lies in linking a defined genetic alteration to observable biological behavior in a controlled human-cell laboratory model.