Crispr Technology
Crispr Technology - Related Videos
01:59
CRISPR
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2019
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
02:53
CRISPR and crRNAs
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2020
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea. The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
10:07
Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology
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Cited by 3 •
2022
This study provides a systematically optimized procedure of CRISPR/Cas9 ribonuclease-based construction of homozygous locust mutants as well as a detailed method for cryopreservation and resuscitation of the locust eggs.
10:52
Precise Phage Mutagenesis with NgTET-Assisted CRISPR-Cas Systems
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2025
Here, we present a protocol to reduce DNA modifications in bacteriophages using the NgTET enzyme, enabling efficient and scarless CRISPR-Cas mutagenesis. This method facilitates the genetic engineering of phages for applications in biotechnology and phage therapy.
11:53
Substrate Generation for Endonucleases of CRISPR/Cas Systems
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Cited by 4 •
2012
CRISPR/Cas systems mediate adaptive immunity in Bacteria and Archaea. Many Cas proteins are proposed to act as endoribonucleases acting on crRNA precursors of varying length. Here we illustrate three different approaches to generate pre-crRNA substrates for the biochemical analysis of Cas endonuclease activity.
FAQs
The protospacer adjacent motif, or PAM, provides a required DNA landmark next to the sequence recognized by the guide RNA. Cas9 uses this neighboring motif to identify a suitable target before making a double-strand break. Consequently, a complementary sequence alone may not be sufficient for editing, because target selection also depends on the presence of an appropriate adjacent PAM.
Guide RNA directs the CRISPR-associated nuclease by carrying a sequence complementary to the intended DNA target. This pairing positions the nuclease at a selected genomic location, where it can cut the DNA. The relationship between guide sequence and target therefore determines editing placement, making guide design central to studying a particular gene or genomic region.
The cell repairs the double-strand break generated by Cas9, and that repair process produces the editing outcome. Depending on the intended experiment, repair can disrupt a gene or support modification of a DNA sequence. Researchers therefore interpret CRISPR results not only from the cutting event itself, but also from how cellular repair changes the targeted genetic information.
A typical workflow selects a DNA region of interest, designs a guide RNA with a complementary sequence, and identifies a compatible adjacent PAM. The guide RNA then directs a CRISPR-associated nuclease, such as Cas9, to that site. After the targeted break occurs, researchers examine the cell's repair outcome to determine whether gene disruption or sequence modification was achieved.
Researchers apply CRISPR technology when they need to connect a gene with a biological role by altering its sequence and examining the resulting consequences. Gene disruption can help reveal what changes when a gene no longer functions normally, while targeted sequence modification can test specific genetic changes. These experiments support functional genomics and broader studies in cell biology.
Its applications extend from functional genomics and disease modeling to crop improvement and investigation of potential therapies. In disease models, targeted genetic changes can help examine biological mechanisms associated with disease. In agriculture and biotechnology, the same adaptable approach supports research on useful traits or engineered biological outcomes, while its precision helps focus experiments on selected DNA regions.