Decellularization Buffer
Decellularization Buffer - Related Videos
09:24
Decellularization and Recellularization of Whole Livers
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Cited by 39 •
2011
Perfusion decellularization is a novel technique to produce whole liver scaffolds that retains the organ's extracellular matrix composition and microarchitecture. Herein, the method of preparing whole organ scaffolds using perfusion decellularization and subsequent repopulation with hepatocytes is described. Functional and transplantable liver grafts can be generated using this technique.
02:56
Buffers
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2020
A solution containing appreciable amounts of a weak conjugate acid-base pair is called a buffer solution, or a buffer. Buffer solutions resist a change in pH when small amounts of a strong acid or a strong base are added. A solution of acetic acid and sodium acetate is an example of a buffer that consists of a weak acid and its salt: CH3COOH (aq) + CH3COONa (aq). An example of a buffer that consists of a weak base and its salt is a solution of ammonia and ammonium chloride: NH3 (aq) + NH4Cl...
04:35
Buffers - Concepts
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2020
Buffers When an Arrhenius acid (HA) is added to water, it dissociates into its conjugate base (A-) and a hydrogen cation (H+). HA + H2O → H+(aq) + A-(aq) The amount of hydrogen ions present in the solution determines the acidity of the solution, where more hydrogen ions indicate a lower or more acidic pH. Similarly, when a strong Arrhenius base (BOH) is added to water, it dissociates into its conjugate acid (B+) and a hydroxide ion (OH-). BOH + H2O → B+(aq) + OH-(aq) Generally, the addition of...
14:31
Buffers - Student Protocol
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2020
Source: Smaa Koraym at Johns Hopkins University, MD, USA Preparing 50 mM NaH2PO4 Buffer, pH 7Expand In the first part of this experiment, you will prepare a sodium phosphate solution buffered at pH 7.0. Monosodium phosphate is a weak acid with the conjugate base, disodium phosphate. Unadjusted monosodium phosphate solutions usually have a pH of about 4 - 6. Buffers are most effective close to their pKa, which is 6.8 to 7.2 for monosodium phosphate. So, you will use NaOH to push the...
08:13
Buffers - Instructor Prep
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2020
Source: Smaa Koraym at Johns Hopkins University, MD, USA Preparation of SolutionsExpand Here, we show the laboratory preparation for 10 students working in pairs, with some excess. Please adjust quantities as needed. To set up for this lab experiment, wear the appropriate personal protective equipment, including a lab coat, chemical splash goggles, and gloves. All solutions for this lab should be prepared in a chemical fume hood. Prepare 100 mL of a 1 M NaOH solution. Note:...
FAQs
Detergents disrupt cell membranes and help solubilize intracellular material, while enzymes can degrade nucleic acids and other cellular components. Salts and additional agents contribute to the chemical environment that supports removal during subsequent washing. Combining these components allows the solution to target cellular material while limiting unnecessary disruption of the extracellular matrix and its associated biochemical cues.
Buffer strength, exposure time, temperature, and perfusion conditions collectively determine the balance between effective cell removal and ECM preservation. More intensive treatment may improve access to cellular material but can also compromise tissue architecture or biochemical cues. Researchers therefore adjust these variables according to the tissue and the desired scaffold properties rather than relying on a single fixed formulation.
The extracellular matrix provides more than physical structure: its retained architecture and biochemical cues support investigation of cell–matrix interactions. If treatment removes cellular material but substantially alters these features, the resulting scaffold may provide less biologically relevant information. Preserving ECM organization therefore helps connect decellularization outcomes with later biomaterial and regenerative research applications.
A typical workflow applies the selected solution to tissue under controlled conditions, allows chemical agents to disrupt and solubilize cellular material, and then uses washing to facilitate removal of the resulting material. Researchers monitor treatment strength and exposure conditions throughout the process, seeking sufficient cell clearance while retaining the tissue’s structural and biochemical ECM characteristics.
Washing helps remove cellular material after it has been disrupted or solubilized by the buffer, while perfusion conditions influence how the solution reaches the tissue. These steps are especially important when researchers need treatment to extend through the biological sample rather than remain limited to accessible surfaces. Their adjustment can improve removal while supporting more uniform preservation of the scaffold.
This approach is useful when researchers need a tissue-derived scaffold for studying cell–matrix interactions or developing biomaterials for regenerative research. The resulting ECM framework can preserve structural and biochemical features that are relevant to those investigations. Accordingly, buffer selection and treatment conditions are evaluated not only by cellular clearance, but also by whether the scaffold remains suitable for the intended study.