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JoVE Journal
Bioengineering

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Decellularization and Recellularization of Whole Livers
 

Decellularization and Recellularization of Whole Livers

Article doi: 10.3791/2394
February 4th, 2011

Summary February 4th, 2011

Perfusion decellularization is a novel technique to produce whole liver scaffolds that retains the organ's extracellular matrix composition and microarchitecture. Herein, the method of preparing whole organ scaffolds using perfusion decellularization and subsequent repopulation with hepatocytes is described. Functional and transplantable liver grafts can be generated using this technique.

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