Dorsal Vessel Observation

Dorsal vessel observation is the direct visualization of the tubular circulatory organ that drives hemolymph circulation in many arthropods, particularly insects. Under a microscope, researchers monitor rhythmic contractions of the dorsal vessel, often through transparent body regions or prepared specimens, to assess heart rate, contraction pattern, and blood flow. In biology, this approach provides a practical way to study insect cardiac function, vascular development, and responses to genetic or chemical manipulation. It supports investigations of cardiovascular physiology and offers an accessible model for examining how circulation changes during development, disease, or exposure to experimental treatments.

Dorsal Vessel Observation - Related Videos

Research

JoVE Journal - Biology

Dissection and Observation of Honey Bee Dorsal Vessel for Studies of Cardiac Function

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Cited by 9 •

2016

The abdominal dorsal vessel of the honey bee and other insects serves as the functional equivalent of the mammalian heart and plays an important role in nutrient transport, waste removal, immune function, and more. Here we describe a protocol for the visualization and pharmacological manipulation of bee heart rate.

Multiphoton Microscopic Observation of Vessels in Mouse Liver Tissue

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2021

In this experiment, a mouse is injected in its tail vein with Rhodamine B isothiocyanate–dextran that can stain blood vessels. After the liver is exposed and fixed, a specific part of the liver can be selected to observe the deep tissue in the living body using multiphoton microscopy.

Education

JoVE Science Education - Psychology

Observational Research

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2023

Source: Laboratories of Gary Lewandowski, Dave Strohmetz, and Natalie Ciarocco—Monmouth University If you want to know how someone thinks or feels, you can ask that person questions. Another approach is to observe how the person is acting or look for indicators of how they acted in the past. While observations may seem revealing, it isn’t always easy to know if they are truly accurate. For instance, you may see a person smiling and assume they are happy, when in reality they are annoyed and...

Dorsal Root Ganglion Injection and Dorsal Root Crush Injury as a Model for Sensory Axon Regeneration

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Cited by 9 •

2017

This protocol presents the use of a dorsal root ganglion (DRG) injection with a viral vector and a concurrent dorsal root crush injury in an adult rat as a model to study sensory axon regeneration. This model is suitable for investigating the use of gene therapy to promote sensory axon regeneration.

Modeling Sensory Axon Regeneration in a Rat Using Dorsal Root Ganglion Injection and Dorsal Root Crush Injury

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2025

Source: Cheah, M., et al. Dorsal Root Ganglion Injection and Dorsal Root Crush Injury as a Model for Sensory Axon Regeneration. J. Vis. Exp. (2017).This video demonstrates a step-by-step procedure for injecting a viral suspension into the dorsal root ganglia (DRGs) and inducing a controlled axonal injury via a dorsal root crush in an anesthetized rat. The protocol involves exposing the DRGs, delivering a growth-promoting viral vector to facilitate axonal regeneration, and severing axonal...

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