Double-stranded Cdna
Double-stranded Cdna - Related Videos
02:04
Fixing Double-strand Breaks
0 Views •
2020
The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
02:04
Fixing Double-strand Breaks
0 Views •
2023
The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
13:46
Separation of Single-stranded DNA, Double-stranded DNA and RNA from an Environmental Viral Community Using Hydroxyapatite Chromatography
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Cited by 5 •
2011
We describe an efficient method to separate single-stranded DNA, double-stranded DNA and RNA molecules from environmental viral communities. Nucleic acids are fractionated using hydroxyapatite chromatography with increasing concentrations of phosphate-containing buffers. This method permits the isolation of all viral nucleic acid types from environmental samples.
05:23
Immunofluorescence Microscopy for the Analysis of DNA Double-Strand Breaks
0 Views •
2025
In this video, we describe the immunofluorescence microscopy technique to detect specific DNA damage response proteins localized at the sites of DNA double-strand breaks in human mononuclear cells. The proteins are recognized by specific primary antibodies, which in turn bind to fluorophore-tagged secondary antibodies that fluoresce during microscopic visualization, enabling visualization of the proteins as distinct fluorescent foci within the cell nuclei.
09:04
Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
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Cited by 12 •
2017
We report the protocols for the synthesis and purification of Peptide Nucleic Acid (PNA) oligomers incorporating modified residues. The biochemical and biophysical methods for the characterization of the recognition of RNA duplexes by the modified PNAs are described.
FAQs
Reverse transcriptase uses messenger RNA as the template for synthesizing a complementary DNA strand. The resulting DNA preserves sequence information from the expressed transcript rather than from the entire genomic region. This initial copy provides the template needed for subsequent formation of the second DNA strand, allowing researchers to convert transcript information into a stable DNA form for downstream analysis.
After the first DNA strand has formed, the RNA template is removed or degraded, leaving the newly synthesized DNA available for copying. A complementary DNA strand can then be produced against that first strand. Completing this paired structure creates a DNA molecule suitable for applications that require a stable, double-stranded representation of expressed genetic information.
Because the template is messenger RNA, the resulting sequence represents expressed gene information without introns. This feature is useful when researchers need a coding sequence for cloning or recombinant protein production, since the DNA reflects the processed transcript rather than the intervening regions present in genomic DNA. It also supports direct comparison of expressed sequences.
A cDNA collection reflects the transcripts present in a particular cell or tissue at the time of sampling. Differences among these DNA copies can therefore provide information about which genes were expressed and how transcript populations vary between biological states or responses. Researchers can use this representation to examine transcript diversity rather than genomic sequence alone.
The workflow begins with messenger RNA serving as the template for reverse transcriptase. This enzyme synthesizes the first DNA strand, after which the RNA template is removed or degraded. The complementary DNA strand is then synthesized against the first strand. The completed product can subsequently be used for library construction, cloning, sequencing, or expression-related studies.
Researchers use it when they need to preserve and analyze information from expressed genes. Common applications include cDNA library construction, cloning, sequencing, gene expression analysis, and recombinant protein production. These uses connect transcript presence with biological conditions, while the double-stranded DNA format provides material that can support several downstream molecular investigations.