Dynamic Sample Loop
Dynamic Sample Loop - Related Videos
08:08
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Closed-loop protocols are becoming increasingly widespread in modern day electrophysiology. We present a simple, versatile and inexpensive way to perform complex electrophysiological protocols in cortical pyramidal neurons in vitro, using a desktop computer and a digital acquisition board.
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Studying DNA Looping by Single-Molecule FRET
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10:50
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17:15
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FAQs
The valve and pump coordinate two distinct phases: loading and injection. During loading, the pump draws sample into the loop while the valve isolates that volume from the flowing system. When the valve changes position, the mobile phase enters the loop and transports the retained sample into the instrument. This sequencing makes delivery controlled and reproducible.
Changing the draw volume or loading conditions changes how much sample the loop can introduce while preserving the same basic valve-controlled sequence. This flexibility is useful when biological workflows require different injection sizes rather than one fixed volume. Consistent control of those settings also helps make separations, detections, or assays more comparable across repeated analyses.
Compared with manual sample transfer, a dynamic sample loop places loading and delivery within a controlled fluid path. The pump and valve perform the transfer without requiring the operator to move the sample between handling steps. In biological analysis, this can reduce manual transfers and improve consistency, supporting more reliable downstream separation, detection, and assay workflows.
An operating sequence begins by positioning the valve to isolate the loop, then using the pump to draw in the selected sample volume. After loading, the valve switches the flow path so mobile phase carries the sample onward into the instrument. The essential controls are loop loading, valve position, and the chosen draw volume.
Chromatography is a major application because the loop can introduce a controlled sample volume into a flowing separation system. The mobile phase then carries the sample into the instrument, supporting reproducible delivery for biological materials. This arrangement helps connect automated sample handling with separation and detection while reducing reliance on repeated manual sample transfers.
In online sampling and assay workflows, the device provides a way to connect sample handling with analysis while the surrounding system remains in flow. Its value extends beyond introducing material: controlled delivery can support process monitoring and analysis of biological samples. The resulting workflow improves consistency while reducing manual handling during automated biological measurements.