Journal
/
Neuroscience
/
Real-time Electrophysiology: Using Closed-loop Protocols to Probe Neuronal Dynamics and Beyond
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Real-time Electrophysiology: Using Closed-loop Protocols to Probe Neuronal Dynamics and Beyond

Real-time Electrophysiology: Using Closed-loop Protocols to Probe Neuronal Dynamics and Beyond

DOI:
10.3791/52320-v

•

08:08 min

•

June 24, 2015

•

, ,
1Department of Biomedical Sciences,University of Antwerp

Chapters

  • 00:05Title
  • 01:43Brain Slice Preparation
  • 02:49Patch-clamp Recordings from Layer 5 Pyramidal Neurons
  • 05:35Injection of Conductance Through Simulated Synapses and Simulation of In Vivo-like Background Activity
  • 06:47Results: Recreation of In Vivo-like Activity Using Dynamic Clamp
  • 07:34Conclusion

Summary

Automatic Translation

Automatic Translation

Closed-loop protocols are becoming increasingly widespread in modern day electrophysiology. We present a simple, versatile and inexpensive way to perform complex electrophysiological protocols in cortical pyramidal neurons in vitro, using a desktop computer and a digital acquisition board.

Tags

Keywords: Real-time ElectrophysiologyClosed-loop ProtocolsNeuronal DynamicsVirtual RealityOptogeneticsNeuronal ExcitabilityReliabilityPatch ClampSomatosensory CortexLCG Software

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/52320?article_type=v

An Experimental Platform to Study the Closed-loop Performance of Brain-machine Interfaces
check_url/52320?article_type=v

Multi-electrode Array Recordings of Neuronal Avalanches in Organotypic Cultures
check_url/52320?article_type=v

Simultaneous Electroencephalography, Real-time Measurement of Lactate Concentration and Optogenetic Manipulation of Neuronal Activity in the Rodent Cerebral Cortex
check_url/52320?article_type=v

Real-Time Impedance-based Cell Analyzer as a Tool to Delineate Molecular Pathways Involved in Neurotoxicity and Neuroprotection in a Neuronal Cell Line
check_url/52320?article_type=v

Closed-loop Neuro-robotic Experiments to Test Computational Properties of Neuronal Networks
check_url/52320?article_type=v

Prolonged Incubation of Acute Neuronal Tissue for Electrophysiology and Calcium-imaging
check_url/52320?article_type=v

Using Neuron Spiking Activity to Trigger Closed-Loop Stimuli in Neurophysiological Experiments
check_url/52320?article_type=v

Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice
check_url/52320?article_type=v

Open-Source Real-Time Closed-Loop Electrical Threshold Tracking for Translational Pain Research
check_url/52320?article_type=v

Therapeutic Benefit of Closed-Loop Deep Brain Stimulation in Depression Treatment (Video) | JoVE

Read Article