Embryo Viability Testing

Embryo viability testing is a set of methods used to estimate whether an embryo can continue normal development and establish a pregnancy. It matters because developmental appearance and biological measurements can help distinguish embryos with greater or lower developmental potential. In practice, laboratories assess cell division, embryo morphology, and progression to the blastocyst stage, sometimes combining these observations with genetic or metabolic analyses. These data support embryo selection for transfer in assisted reproduction and provide experimental measures for studying early development, causes of developmental failure, and the effects of culture conditions.

Embryo Viability Testing - Related Videos

Research

JoVE Journal - Developmental Biology
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A Direct and Simple Method to Assess Drosophila melanogaster's Viability from Embryo to Adult

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Cited by 6 •

2019

This protocol is designed to assess the viability of Drosophila at every developmental stage, from embryo to adult. The method can be used to determine and compare the viability of different genotypes or growth conditions.

Research

JoVE Journal - Biology

Viability Assays for Cells in Culture

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Cited by 39 •

2014

Therapeutic compounds are often first examined in vitro with viability assays. Blind cell counts by a human observer can be highly sensitive to small changes in cell number but do not assess function. Computerized viability assays, as described here, can assess both structure and function in an objective manner.

Counting and Determining the Viability of Cultured Cells

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Cited by 19 •

2008

Determining the number of cells in culture is important in standardization of culture conditions and in performing accurate quantitation experiments. In this video, we demonstrate how cells are counted using a hemacytometer.

Fluorescence-Based Viability Assessment of Oral Anaerobic Bacteria

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2026

Source: Calatayud Arroyo, M., et al. Assessing the Viability of a Synthetic Bacterial Consortium on the In Vitro Gut Host-microbe Interface. J. Vis. Exp. (2018)The video demonstrates a method to evaluate the viability of anaerobic oral bacteria using fluorescent dyes and flow cytometry. It begins with culturing bacteria in oxygen-free broth, followed by resuspending a single colony from a blood agar plate. After incubation and dilution, the culture is stained with membrane-permeable and...

Viability of Bioprinted Cellular Constructs Using a Three Dispenser Cartesian Printer

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Cited by 6 •

2015

A Cartesian bioprinter was designed and fabricated to allow multi-material deposition in precise, reproducible geometries, while also allowing control of environmental factors. Utilizing the three-dimensional bioprinter, complex and viable constructs may be printed and easily reproduced.

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