Endocytic Rate Measurement
Endocytic Rate Measurement - Related Videos
11:32
Measuring Plasma Membrane Protein Endocytic Rates by Reversible Biotinylation
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Cited by 23 •
2009
Regulated endocytosis governs the cell surface expression levels of the majority of membrane proteins. Here we utilize reducible, membrane impermeant biotinylation reagents to measure the endocytic rate of the dopamine transporter (DAT), a polytopic membrane protein. The method facilitates a straightforward approach to measuring the endocytic rate of most plasma membrane proteins.
03:09
Measuring Reaction Rates
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2020
Polarimetry finds application in chemical kinetics to measure the concentration and reaction kinetics of optically active substances during a chemical reaction. Optically active substances have the capability of rotating the plane of polarization of linearly polarized light passing through them—a feature called optical rotation. Optical activity is attributed to the molecular structure of substances. Normal monochromatic light is unpolarized and possesses oscillations of the electrical field in...
08:32
Measurement of Outgassing Rates of Steels
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Cited by 5 •
2016
A protocol for the measurement of outgassing rates of hydrogen from ordinary steel vacuum chambers using the rate-of-pressure rise method is presented.
10:42
Determining Cell-surface Expression and Endocytic Rate of Proteins in Primary Astrocyte Cultures Using Biotinylation
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Cited by 6 •
2017
Two biotinylation-based methods, designed for determining the cell-surface expression and endocytic rate of proteins expressed at the plasma membrane, are presented in this report.
13:35
Mass Conservation and Flow Rate Measurements
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2023
Source: Ricardo Mejia-Alvarez and Hussam Hikmat Jabbar, Department of Mechanical Engineering, Michigan State University, East Lansing, MI The purpose of this experiment is to demonstrate the calibration of a flow passage as a flowmeter using a control volume (CV) formulation [1, 2]. The CV analysis focuses on the macroscopic effect of flow on engineering systems, rather than the detailed description that could be achieved with a detailed differential analysis. These two techniques should be...
FAQs
The assay requires a separation between cargo that has entered the cell and cargo still associated with the plasma membrane. Researchers expose cells to measurable or fluorescent material, then distinguish these two pools before quantifying uptake over a defined interval. This distinction prevents surface-associated signal from being interpreted as internalization and makes comparisons of cellular uptake rates possible.
Comparing receptor-mediated uptake with fluid-phase uptake separates cargo-selective behavior from less selective sampling of extracellular fluid. A change in the receptor-mediated component can indicate altered handling of a specific cargo, whereas a change in fluid-phase uptake reflects a broader shift in internalization. Measuring both modes therefore gives a more informative picture than treating all endocytosis as one process.
The defined exposure interval provides the time basis for calculating how much material cells internalize. Using a measurable or fluorescent cargo during that interval allows researchers to relate the amount entering cells to elapsed time and compare uptake between cellular conditions. The timing also helps identify changes in endocytic activity caused by drugs, mutations, or disease-related states.
Cells first receive a measurable or fluorescent cargo for a specified period. The assay then distinguishes material internalized by the cells from material that remains associated with the plasma membrane. Researchers quantify the internalized portion and use the defined uptake interval to assess endocytic rate. Applying the same approach across conditions enables direct comparison of cellular internalization.
Researchers use this approach when they need a quantitative assessment of membrane or cargo uptake over time. It can compare untreated and drug-exposed cells, examine effects of mutations, or evaluate differences associated with disease. The resulting measurements help determine whether cellular changes affect receptor regulation, nutrient uptake, membrane balance, or broader trafficking behavior.
By quantifying internalization, the method connects endocytic activity with processes that depend on movement of plasma-membrane components and extracellular cargo. It can help characterize receptor regulation, nutrient uptake, membrane balance, and cellular responses. In biology, these measurements provide a way to relate altered uptake rates to changes in signaling or membrane-trafficking behavior under different cellular conditions.