Endophytic Bacteria

Endophytic bacteria are microorganisms that live within healthy plant tissues without causing apparent disease, making them important partners in plant biology and agricultural research. They enter through roots, wounds, or natural openings and colonize internal spaces, where they interact with plant cells and exchange chemical signals, nutrients, and growth-regulating compounds. These associations can promote plant development, improve nutrient acquisition, enhance tolerance to drought or other stresses, and suppress pathogens through antimicrobial activity or competition. Studying endophytic bacteria supports the development of biological fertilizers, sustainable crop-protection strategies, and microbial treatments that improve plant health while reducing reliance on synthetic inputs.

Endophytic Bacteria - Related Videos

Research

JoVE EoE - Bacterial Growth and Techniques

Root-Mediated Endophytic Colonization of Beneficial Bacteria in Rice

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2025

Begin with rice seedlings pre-germinated under dark, aseptic conditions to ensure uniform sprouting.Transfer these seedlings to a flask containing a nutrient-rich, semi-solid medium pre-inoculated with a test bacterium.As the plant grows, the roots release chemotactic compounds into the surrounding medium.These compounds stimulate bacterial proliferation, leading to adhesion, multiplication, and colony formation on the root surface.As the roots develop, the bacteria enter the plant through...

Research

JoVE Journal - Bioengineering
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Establishing Fungal Entomopathogens as Endophytes: Towards Endophytic Biological Control

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Cited by 197 •

2013

This protocol demonstrates two inoculation methods to introduce the fungal entomopathogen Beauveria bassiana as an endophyte in the common bean (Phaseolus vulgaris), in preparation for subsequent evaluations of endophytic biological control.

Enhancing Salt Stress Tolerance in Tomato Plants Through Endophytic Bacterial Colonization

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2025

Endophytic bacteria colonize plant tissues and promote plant growth.To study their impact on tomato plant growth under salt stress, start with sterile seedlings showing developing leaves and roots.Immerse the roots of the experimental seedling in a suspension of endophytic bacteria; leave the control uninoculated.Transfer both groups into sterilized soil to prevent external microbial interference and incubate in a growth chamber.From the soil, the bacteria enter through transient root openings,...

Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants

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Cited by 2 •

2023

The current article aims to provide detailed and adequate protocols for the isolation of plant-associated endophytic fungi, long-term preservation of isolates, morphological characterization, and total DNA extraction for subsequent molecular identification and metagenomic analyses.

Education

JoVE Science Education - Environmental Sciences

Culturing and Enumerating Bacteria from Soil Samples

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2023

Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba - The University of Arizona Demonstrating Authors: Bradley Schmitz and Luisa Ikner Surface soils are a heterogeneous mixture of inorganic and organic particles that combine together to form secondary aggregates. Within and between the aggregates are voids or pores that visually contain both air and water. These conditions create an ideal ecosystem for bacteria, so all soils contain vast populations of bacteria, usually over 1 million...

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