Exosomal Micrornas
Exosomal Micrornas - Related Videos
06:59
Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile
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Cited by 17 •
2016
Bile fluid is a valuable source of extracellular vesicles/exosomes that contain potentially important biomarkers. This protocol represents a robust method to isolate exosomes from human bile for further analyses including miRNA profiling.
10:45
Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media
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Cited by 45 •
2013
The presence of stable microRNAs (miRNAs) in exosomes has generated immense interest as a novel mode of intercellular communication, for their potential utility as biomarkers and as a route for therapeutic intervention. Here we demonstrate exosome purification from blood and culture media followed by quantitative PCR to identify miRNAs being transported.
04:21
Exosome Isolation: A Technique to Separate Exosomes from the Plasma of Non-small Cell Lung Cancer Patients
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2023
The plasma-isolated exosomes can be used as a non-invasive and repeatable way for the detection and follow-up of the biomarkers for early detection and disease progression. This video describes the protocol for the separation of exosomes from the plasma samples of non-small cell lung cancer patients, which could be used for DNA and protein extractions.
01:22
MicroRNAs
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2019
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
07:45
Tear-Derived Exosomal miR-15a as New Diagnostic Tool for Diabetic Retinopathy
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Cited by 1 •
2025
This article describes a protocol for the collection and detection of miR-15a from tears as a new diagnostic tool for diabetic retinopathy.
FAQs
Once a recipient cell takes up an exosome, its microRNA cargo can recognize messenger RNAs through complementary sequence pairing. This interaction may promote degradation of the targeted transcript or inhibit its translation, reducing the production of the corresponding protein. The effect provides a molecular route through which one cell can influence gene expression and behavior in another.
Packaging places the regulatory cargo inside a small extracellular vesicle that can be released and taken up by another cell. Exosomal microRNAs can also remain stable in biofluids, which makes them accessible for studying signals outside the cells that produced them. These properties connect intercellular communication with biomarker research and help explain their biological and clinical interest.
Uptake is the transition that brings the cargo into the recipient cell, where its regulatory activity can be expressed. Before that step, the microRNA remains associated with the released vesicle rather than interacting with the recipient cell’s messenger RNAs. Consequently, studies of signaling must consider both exosome release and delivery, not cargo presence alone.
The source material links this signaling to development, immune responses, metabolism, and disease progression. These examples show that exosomal microRNAs participate in biological communication across both normal and disease-related contexts. In biology research, examining this cargo can therefore help connect extracellular signaling with changes in gene regulation and cellular function.
Their stability in biofluids makes exosomal microRNAs suitable for investigation as measurable molecular indicators. Researchers study these signals for diagnostic applications and prognostic assessment, using them to relate extracellular molecular patterns to biological or disease-related states. This approach is especially valuable when the goal is to evaluate disease progression rather than only examine intracellular gene regulation.
Beyond biomarker research, exosomal microRNAs are investigated as potential tools for delivering regulatory signals to recipient cells. Their ability to influence messenger RNAs gives this approach a mechanistic basis for therapeutic applications. The topic also has regenerative relevance, because researchers study exosome-mediated signaling in the context of regenerative applications rather than solely as a marker of disease.