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Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media
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Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media

Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media

DOI:
10.3791/50294-v

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10:45 min

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June 14, 2013

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, ,
1Department of Pharmacology & Physiology,Drexel University College of Medicine

Chapters

  • 00:05Title
  • 01:37Exosome Purification from Blood
  • 03:41miRNA Profiling of Exosomes from Blood Using Taqman Low Density Array (TLDA) Cards
  • 08:38Results: Exosome RNA and microRNA Levels Offer a Unique Platform for Biomarker Discovery
  • 10:16Conclusion

Summary

Automatic Translation

Automatic Translation

The presence of stable microRNAs (miRNAs) in exosomes has generated immense interest as a novel mode of intercellular communication, for their potential utility as biomarkers and as a route for therapeutic intervention. Here we demonstrate exosome purification from blood and culture media followed by quantitative PCR to identify miRNAs being transported.

Tags

Keywords: ExosomesMicroRNAMiRNA ProfilingPurificationBloodCell Culture MediaBiomarkersGene ExpressionQPCRTaqman Low Density ArrayTEMImmunogold Labeling

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