Ffpe Biopsy Tissue
Ffpe Biopsy Tissue - Related Videos
02:43
Deparaffinization and Rehydration of Tissue Sections: A Two-step Procedure to Remove Paraffin Wax from FFPE Tissue Slides Followed by Tissue Rehydration
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2023
In this video, we demonstrate the processing of FFPE or formalin-fixed paraffin-embedded sections obtained from gastrointestinal tumor tissue. The deparaffinization and rehydration steps allow the processing of the tissue specimen for subsequent analytical applications.
04:17
Formalin-Fixed Paraffin Embedded (FFPE) Tissue Preservation: A Method for Studying Tissue Morphology
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2023
The following video describes how to preserve morphology of a tissue with formalin fixation and paraffin embedding.
04:33
Extracellular DNA Staining: A Method to Identify Extracellular DNA in FFPE Tissue Sections
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2023
This video describes the technique of staining extracellular DNA in FFPE tissue sections. The stained extracellular DNA can be quantitated to determine the efficacy of any therapeutic treatment that leads to the death of cancer cells.
08:08
qPCR Is a Sensitive and Rapid Method for Detection of Cytomegaloviral DNA in Formalin-fixed, Paraffin-embedded Biopsy Tissue
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Cited by 4 •
2014
This protocol describes qPCR detection of cytomegalovirus in formalin-fixed, paraffin-embedded biopsy tissue, which is rapid, sensitive, specific, and useful for interpreting equivocal hematoxylin and eosin or immunohistochemical staining patterns.
06:33
Establishment of Human Epithelial Enteroids and Colonoids from Whole Tissue and Biopsy
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Cited by 132 •
2015
We describe a method to establish human enteroids from small intestinal crypts and colonoids from colon crypts collected from both surgical tissue and biopsies. In this methodological article, we present the culture modalities that are essential for the successful growth and maintenance of human enteroids and colonoids.
FAQs
Formalin stabilizes the specimen by cross-linking cellular proteins, helping preserve tissue architecture during later processing. This structural preservation allows pathologists and researchers to examine relationships among cells and tissue compartments rather than viewing a disrupted sample. The resulting morphology supports histological staining, microscopic examination, and immunohistochemistry, where preserved architecture helps place molecular or cellular signals in tissue context.
Dehydration removes water from the biopsy, preparing it for infiltration with molten paraffin. Once the paraffin fills the tissue and solidifies, it provides a firm support matrix that can be cut into thin sections. This mechanical stability is essential for producing consistent slices suitable for staining and microscopy, while maintaining the preserved tissue architecture established during fixation.
A single preserved specimen can provide complementary biological information. Thin sections reveal tissue organization and cellular features through staining and microscopy, while material from the block can support DNA or RNA extraction for selected molecular assays. Using these perspectives together connects visible tissue changes with molecular findings, helping characterize disease features and investigate biomarkers in archived samples.
The workflow progresses from formalin fixation to dehydration, paraffin infiltration, and formation of a solid block. The block is then sectioned into thin slices for staining and microscopic examination. Depending on the study, preserved material may also be used for DNA or RNA extraction. This sequence converts a biopsy into a stable format for repeated histological or selected molecular analyses.
Thin sections make preserved tissue architecture accessible to visual analysis. After staining, researchers or pathologists can examine cellular and structural features under a microscope, while immunohistochemistry can add information about specific tissue markers. These observations support routine diagnosis and can contribute to investigations of disease features, biomarker patterns, and treatment responses in stored specimens.
Archived blocks retain clinically valuable information and can be revisited for retrospective studies. Investigators can examine disease features, assess biomarker findings, or study treatment responses without relying only on newly collected biopsies. The same archive may therefore support both microscopic evaluation and selected molecular assays, extending the research value of specimens beyond their original diagnostic use.