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qPCR Is a Sensitive and Rapid Method for Detection of Cytomegaloviral DNA in Formalin-fixed, Paraffin-embedded Biopsy Tissue
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qPCR Is a Sensitive and Rapid Method for Detection of Cytomegaloviral DNA in Formalin-fixed, Paraffin-embedded Biopsy Tissue

qPCR Is a Sensitive and Rapid Method for Detection of Cytomegaloviral DNA in Formalin-fixed, Paraffin-embedded Biopsy Tissue

DOI:
10.3791/51570-v

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08:08 min

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July 09, 2014

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, , , , , , , , ,
1Department of Pathology and Laboratory Medicine,Indiana University School of Medicine, 2Department of Pathology and Laboratory Medicine,Indiana University Health

Chapters

  • 00:05Title
  • 01:24Processing FFPE GI Tissue Blocks
  • 03:17DNA Extraction from Tissue Scrolls
  • 04:30Quantitative PCR
  • 06:39qPCR Results on Control Tissues
  • 07:41Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes qPCR detection of cytomegalovirus in formalin-fixed, paraffin-embedded biopsy tissue, which is rapid, sensitive, specific, and useful for interpreting equivocal hematoxylin and eosin or immunohistochemical staining patterns.

Tags

Keywords: QPCRCytomegalovirusCMVImmunosuppressedGastrointestinal TractFFPEBiopsyHistologyH&EImmunohistochemistryIHCMolecular DetectionSensitivitySpecificity

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