Field Diaphragm
Field Diaphragm - Related Videos
07:12
Diagnostic Ultrasound Imaging of Mouse Diaphragm Function
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Cited by 7 •
2014
Diagnostic ultrasound imaging has proven to be effective in diagnosing various respiratory diseases in human and animal subjects. We demonstrate a comprehensive ultrasound protocol utilized by Dr. Zuo's lab to analyze diaphragm kinetics specifically in mouse models. This is also a non-invasive research technique which can provide quantitative information on mouse respiratory muscle function.
09:43
Functional and Morphological Assessment of Diaphragm Innervation by Phrenic Motor Neurons
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Cited by 16 •
2015
Compound muscle action potential recording quantitatively assesses functional diaphragm innervation by phrenic motor neurons. Whole-mount diaphragm immunohistochemistry assesses morphological innervation at individual neuromuscular junctions. The goal of this protocol is to demonstrate how these two powerful methodologies can be used in various rodent models of spinal cord disease.
02:48
Fluorescence Imaging of Calcium Dynamics at Neuromuscular Junctions in the Diaphragm of a Transgenic Mouse
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2025
Source: Heredia, D. J., et. al. Ex Vivo Imaging of Cell-specific Calcium Signaling at the Tripartite Synapse of the Mouse Diaphragm. J. Vis. Exp. (2018) This video demonstrates fluorescence imaging of calcium dynamics at neuromuscular junctions (NMJs) in a transgenic mouse expressing genetically encoded calcium indicators. A NJM is identified by fluorescently tagged acetylcholine receptors in the diaphragm with the phrenic nerve. An image splitter is used to visualize multiple signals...
05:51
Measuring Diaphragm Thickness and Function Using Point-of-Care Ultrasound
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Cited by 9 •
2023
Diaphragm thickness and function can be assessed in healthy individuals and critically ill patients using point-of-care ultrasound. This technique offers an accurate, reproducible, feasible, and well-tolerated method for evaluating diaphragm structure and function.
10:23
Electric Fields
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2023
Source: Yong P. Chen, PhD, Department of Physics & Astronomy, College of Science, Purdue University, West Lafayette, IN An electric field is generated by a charged object (referred to as the source charge) in the space around it, and represents the ability to exert electric force on another charged object (referred to as the test charge). Represented by a vector at any given point in the space, the electric field is the electrical force per unit test charge placed at that point (the force...
FAQs
In Köhler illumination, the field diaphragm and condenser must be considered together: the iris aperture limits the illuminated area, while the condenser helps focus light evenly onto that field. Their coordinated use prevents illumination from extending unnecessarily beyond the viewed region. The result is a more controlled optical field for examining biological specimens.
Restricting the light field to the viewed area reduces stray light that could otherwise enter the image from outside the region of interest. This control can improve contrast, making boundaries and other visual features easier to assess. It is especially useful when biological observations depend on distinguishing specimen detail from unwanted illumination.
By adjusting the iris blades and observing the resulting field, a microscopist can assess whether an apparent feature follows the specimen or arises from illumination. Limiting the field reduces stray light and supports more even illumination, so uneven brightness or other artifacts are less likely to be mistaken for cellular, tissue, or microbial structures.
Adjustment requires a microscope’s illumination system, the field diaphragm’s iris blades, and the condenser used for Köhler illumination. The operator changes the aperture while viewing the specimen, then evaluates whether the light field corresponds to the area being observed. This setup makes the adjustment relevant to both illumination uniformity and contrast in bright-field work.
During bright-field viewing, adjust the iris blades so illumination remains confined to the area being examined, while using the condenser as part of Köhler illumination. Observe whether the field appears evenly illuminated and whether stray light is reduced. This provides a practical check that the lighting supports clearer specimen observation rather than introducing distracting illumination effects.
Cells, tissues, and microorganisms can all benefit from a well-adjusted field diaphragm because illumination can be confined to the area being examined. In bright-field microscopy, this supports clearer observation and helps the viewer evaluate whether visible patterns belong to the specimen rather than the lighting conditions. The adjustment is therefore relevant across several common biological sample types.
A suitable setting can produce a more uniformly illuminated field, which supports clearer photodocumentation of cells, tissues, and microorganisms. By limiting unnecessary illumination and reducing stray light, the field diaphragm can help images represent specimen features more consistently. This is valuable when photographs are used to record observations or compare visible biological structures.