Flow Chamber Imaging
Flow Chamber Imaging - Related Videos
12:42
Human Neutrophil Flow Chamber Adhesion Assay
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Cited by 12 •
2014
A method of quantitating neutrophil adhesion is reported. This method creates a dynamic flow environment similar to that encountered in a blood vessel. It allows the investigation of neutrophil adhesion to either purified adhesion molecules (ligand) or endothelial cell substrate (HUVEC) in a context similar to the in vivo environment with sheer stress.
12:29
Upright Imaging of Drosophila Egg Chambers
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Cited by 1 •
2015
The upright imaging method described in this protocol allows for the detailed visualization of the poles of a developing Drosophila melanogaster egg. This end-on view provides a new perspective into the arrangements and morphologies of multiple cell types in the follicular epithelium.
12:43
Parallel-plate Flow Chamber and Continuous Flow Circuit to Evaluate Endothelial Progenitor Cells under Laminar Flow Shear Stress
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Cited by 51 •
2012
We are describing a method to subject adherent cells to laminar flow shear stress in a sterile continuous flow circuit. The cells' adhesion, morphology can be studied through the transparent chamber, samples obtained from the circuit for metabolite analysis and cells harvested after shear exposure for future experiments or culture.
10:56
A Novel Three-dimensional Flow Chamber Device to Study Chemokine-directed Extravasation of Cells Circulating under Physiological Flow Conditions
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Cited by 7 •
2013
The three-dimensional flow chamber device is a novel in vitro technology for the quantitative and step-wise evaluation of the extravasation cascade of cells circulating under conditions of physiological shear stress. The device therefore fills a critical need for basic, preclinical, and clinical studies of cell migration.
10:38
Preparing Individual Drosophila Egg Chambers for Live Imaging
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Cited by 19 •
2012
The Drosophila egg chamber is an excellent model for studying the mechanisms of mRNA localization. In order to capture the dynamic events that underpin the processes of localization, rapid high resolution imaging of live tissue is required. Here, we present a protocol for dissection and imaging of live samples with minimal disruption.
FAQs
Changing the pump-driven flow rate alters the shear forces experienced by cells, particles, or microorganisms inside the chamber. This controlled adjustment helps researchers examine behavior under different fluid conditions rather than a single static environment. Comparing responses across flow settings can reveal how movement, adhesion, deformation, or surface interactions depend on mechanical conditions.
Real-time image capture allows researchers to follow cellular or microbial behavior as it occurs during fluid movement. Instead of relying only on an endpoint measurement, they can observe changes in adhesion, migration, deformation, or interactions with a surface. This temporal information supports quantitative analysis of dynamic behavior that static culture systems cannot provide.
The technique can reveal several distinct responses, including whether cells attach to a surface, migrate along it, deform during movement, or interact with other surfaces or materials. Observing these behaviors within the same controlled fluid environment helps separate movement-related responses from behaviors that appear only in stationary culture.
Static culture does not reproduce the defined fluid movement and adjustable shear forces created within the chamber. Flow Chamber Imaging therefore provides a way to study behavior under conditions that better represent physiological fluid movement. The comparison is especially useful when adhesion, trafficking, deformation, or surface interactions may change in response to flow.
A typical workflow places cells, particles, or microorganisms in a narrow chamber, connects the chamber to a pump, establishes a selected liquid flow, and captures microscopy images while movement occurs. Researchers then compare the observed behavior under the chosen flow conditions, focusing on measurable changes such as adhesion, migration, deformation, or surface interaction.
The pump-driven liquid flow rate is a central controllable variable, and adjusting it changes the shear forces within the chamber. Researchers can therefore examine responses across defined fluid conditions while imaging the same type of moving subject. This control supports quantitative comparisons of behavior rather than observations made under uncontrolled movement.
Its applications include studying vascular function, immune-cell trafficking, microbial attachment, and interactions between cells and engineered or other material surfaces. These cases share a need to examine biological behavior during fluid movement. By providing images and quantitative behavioral measurements, the method connects cellular responses with flow conditions relevant to biological systems.