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JoVE Journal
Immunology and Infection

This content is Open Access.

Human Neutrophil Flow Chamber Adhesion Assay

Human Neutrophil Flow Chamber Adhesion Assay

Article DOI: 10.3791/51410 12:42 min
July 2nd, 2014


Summary July 2nd, 2014

A method of quantitating neutrophil adhesion is reported. This method creates a dynamic flow environment similar to that encountered in a blood vessel. It allows the investigation of neutrophil adhesion to either purified adhesion molecules (ligand) or endothelial cell substrate (HUVEC) in a context similar to the in vivo environment with sheer stress.

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