Fluid Uptake

Fluid uptake is the movement of water and dissolved substances into cells, tissues, or organisms, supporting hydration, nutrient acquisition, and waste management. In biology, it occurs through processes such as osmosis, membrane transport, and pinocytosis, in which concentration or water-potential gradients drive movement across selectively permeable membranes, while cells may engulf extracellular fluid within small vesicles. Fluid uptake helps maintain cellular volume and internal conditions and contributes to nutrient absorption in tissues such as the intestine and roots. Measuring these processes is important in studies of cell physiology, transport mechanisms, plant function, and disease-related changes in tissue hydration.

Fluid Uptake - Related Videos

Research

JoVE Journal - Biology

The Ingestion of Fluorescent, Magnetic Nanoparticles for Determining Fluid-uptake Abilities in Insects

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Cited by 3 •

2017

Fluid-feeding insects have the ability to acquire minute quantities of liquids from porous surfaces. This protocol describes a method to directly determine the ability for insects to ingest liquids from porous surfaces using feeding solutions with fluorescent, magnetic nanoparticles.

Radiolabeled Amino Acid Uptake Assay to Quantify Cellular Uptake of Amino Acids

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2025

This video demonstrates an in vitro technique for estimating amino acid uptake by bone marrow-derived stromal cells. Na+-dependent amino acid transporters mediate the uptake of radiolabeled amino acids inside the cells, and the cellular uptake is estimated by measuring the radioactivity.

Measuring Dopamine Uptake in Mouse Brain Synaptosomes Using Radiolabeled Dopamine

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2025

This video demonstrates the assessment of dopamine uptake in synaptosomes harvested from mouse brains. It outlines steps for preparing synaptosomes for dopamine uptake via dopamine transporters, treating them with varying concentrations of radiolabeled dopamine, isolating the synaptosomes, and measuring radioactivity to quantify dopamine uptake.

Mitochondrial Calcium Uptake Assay: A Plate Reader-Based Method for Measuring Calcium Uptake in Isolated Mitochondria

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2025

This video describes a plate reader-based assay to measure mitochondrial calcium uptake in isolated mitochondria. The assay analyzes the fluorescence signal of a calcium-sensitive fluorescent dye to study the kinetics of mitochondrial calcium uptake and calcium overload.

Competence-Induced DNA Uptake in Staphylococcus aureus

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2025

Source: Cafini, F., et.al. Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus. J. Vis. Exp. (2017)This video demonstrates competence-induced DNA uptake in Staphylococcus aureus through natural transformation. A chloramphenicol-sensitive strain engineered to constitutively express a sigma factor is cultured under nutrient-limiting conditions to enhance competence, then incubated with donor DNA carrying a resistance gene. Successful gene transfer is confirmed by the...

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