Fluorescent Colony Detection
Fluorescent Colony Detection - Related Videos
09:10
Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce
0 Views •
Cited by 17 •
2010
This protocol describes a simple adhesive-tape-based approach for sampling of tomato and other fresh produce surfaces, followed by rapid whole cell detection of Salmonella using fluorescence in situ hybridization (FISH).
06:13
Colony Formation Assay Detecting the Proliferative Capacity of LncRNA-knockdown Osteosarcoma Cells
0 Views •
2026
This experimental protocol provides a stable, cost-effective, and efficient method that combines lncRNA knockdown with colony-forming assays to quantify the effect of lncRNA on osteosarcoma cell proliferation.
06:44
Keratinocyte Colony Formation Assay: An In Vitro Assay to Assess the Ability of Keratinocytes to Grow into Colonies
0 Views •
2023
In this video, we perform a clonogenic assay of keratinocytes harvested from adult mice to determine the ability of individual cells to proliferate and form colonies. This keratinocyte harvesting technique is useful for studying other downstream aspects of cutaneous chemical carcinogenesis and tumor promotion.
03:44
Detection of Inflammasome Activation via Fluorescence Microscopy
0 Views •
2025
This video demonstrates a method to detect NLRP3 inflammasome activation in macrophages using fluorescent reporter probes. The cells are treated with potassium ionophores and glycine to induce inflammasome assembly and caspase-1 activation. Fluorescent reporter probes visualize activated caspase-1, and cells are stained with a fluorescent dye for nuclei visualization.
14:16
Fluorescence detection methods for microfluidic droplet platforms
0 Views •
Cited by 20 •
2011
Droplet-based microfluidic platforms are promising candidates for high throughput experimentation since they are able to generate picoliter, self-compartmentalized vessels inexpensively at kHz rates. Through integration with fast, sensitive and high resolution fluorescence spectroscopic methods, the large amounts of information generated within these systems can be efficiently extracted, harnessed and utilized.
FAQs
The fluorescent marker must first absorb an appropriate excitation wavelength before emitting detectable light. An imaging system then records the resulting emission, while surrounding media may contribute background fluorescence. Selecting conditions that separate marker emission from background helps make colonies easier to locate and distinguish, improving the reliability of signal-based analysis.
Signal intensity provides a measurable indication of fluorescence associated with a colony, whereas spatial patterns show where fluorescent cells are distributed. Comparing these features with background fluorescence helps distinguish meaningful colony signals from surrounding growth media. Together, they support more consistent identification and can provide quantitative information rather than relying only on visual inspection.
Distinguishability depends on the relationship between cellular fluorescence and background fluorescence. Stronger separation between the emitted signal and the surrounding medium generally makes colony locations and patterns easier to identify. Imaging conditions and the distribution of fluorescent cells also influence the recorded result, so interpretation should consider both signal intensity and spatial organization.
A typical workflow begins by examining microbial growth with illumination that excites the fluorescent marker. The emitted light is captured by an imaging system, and the resulting image is evaluated for signal intensity or spatial patterns. Researchers then compare these measurements with background fluorescence to identify colonies and select those matching the experimental criteria.
The method is useful when researchers need to screen transformed microorganisms or identify colonies associated with a desired genetic construct. Fluorescent signals can help locate candidate colonies among surrounding growth and support selection based on measurable patterns. This makes the approach relevant to microbial genetics, biotechnology studies, and experiments requiring efficient colony screening.
Fluorescent colony detection can increase screening throughput by helping researchers locate and evaluate colonies through imaging rather than visual searching alone. Because signal intensity and spatial patterns can be analyzed quantitatively, the method also supports assay development and monitoring of colony formation. These measurements provide additional information for comparing growth outcomes or selecting strains.