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Fluorescence detection methods for microfluidic droplet platforms
JoVE Journal
Bioengineering
Author Produced
This content is Free Access.
JoVE Journal Bioengineering
Fluorescence detection methods for microfluidic droplet platforms
DOI:

14:16 min

December 10, 2011

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Chapters

  • 00:05Title
  • 00:27Introduction
  • 02:12Microchip Fabrication
  • 04:07Microfluidic Setup
  • 05:55Optical Setup
  • 08:00Data Acquisition
  • 09:00Cell Recognition
  • 11:13Mapping of Mixing Events Using FLIM
  • 13:04Conclusion

Summary

Automatic Translation

Droplet-based microfluidic platforms are promising candidates for high throughput experimentation since they are able to generate picoliter, self-compartmentalized vessels inexpensively at kHz rates. Through integration with fast, sensitive and high resolution fluorescence spectroscopic methods, the large amounts of information generated within these systems can be efficiently extracted, harnessed and utilized.

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