Follicular Cells
Follicular Cells - Related Videos
06:40
Collection of Human Follicular Fluid, Follicle Somatic Cells, and Immature Oocytes from Individuals Undergoing In Vitro Fertilization
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2025
This protocol outlines standardized methods to isolate and process follicular fluid, somatic cells, and immature oocytes from IVF procedures, enabling high-quality molecular and cellular analyses. These approaches support translational research into fertility, reproductive aging, and ovarian dysfunction using materials typically discarded in clinical care.
07:24
Xenopus Oocytes: Optimized Methods for Microinjection, Removal of Follicular Cell Layers, and Fast Solution Changes in Electrophysiological Experiments
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Cited by 5 •
2016
Optimized procedures for the isolation of single follicles, cytoplasmic RNA microinjections, the removal of surrounding cell layers, and protein expression in Xenopus oocytes are described. In addition, a simple method for fast solution changes in electrophysiological experiments with ligand-gated ion channels is presented.
11:35
Separation of Follicular Cells and Oocytes in Ovarian Follicles of Zebrafish
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Cited by 1 •
2021
Here, we present a simple method for separating follicular cells and oocytes in zebrafish ovarian follicles, which will facilitate investigations of ovarian development in zebrafish.
07:07
Evaluation of T Follicular Helper Cells and Germinal Center Response During Influenza A Virus Infection in Mice
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Cited by 2 •
2020
This paper describes protocols of evaluating Tfh and GC B response in mouse model of influenza virus infection.
05:03
Accessing Early Differentiation of Virus-Specific Follicular Helper CD4+ T Cell in Acute LCMV-Infected Mice
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2024
The current study showcases protocols for assessing the early fate commitment of virus-specific TFH cells and manipulating gene expression in these cells.
FAQs
Thyroid-stimulating hormone (TSH) acts as a regulatory signal for follicular cells. In response, these cells control the iodination of thyroglobulin and the subsequent release of thyroid hormones T3 and T4. This link between hormonal signaling and cellular activity explains how thyroid tissue adjusts hormone production within its follicles.
Colloid serves as the follicular lumen’s storage environment for thyroglobulin, the protein made by thyroid follicular cells. Its presence separates synthesis from later hormone release, while iodination within this organized follicular system supports production of T3 and T4. Examining colloid therefore helps relate follicle structure to endocrine function.
The follicular wall organizes exchange between the bloodstream and the lumen rather than allowing these compartments to mix freely. This spatial separation supports controlled handling of thyroglobulin, iodination, storage, and hormone release. Consequently, tissue architecture is not merely structural; it is part of the regulatory system that links circulating signals with thyroid output.
Histological analysis links microscopic organization with thyroid function. By examining follicle arrangement, lumen contents, and the relationship between cells and colloid, investigators can evaluate how tissue structure supports hormone synthesis and storage. The same approach provides a basis for studying pathological changes associated with goiter or thyroid cancer, without relying only on hormone measurements.
Cell culture models allow investigators to study follicular cells under controlled experimental conditions while focusing on hormone-related processes. They can be used to investigate thyroglobulin synthesis, responses associated with TSH, iodination, and release of T3 and T4. These models complement tissue studies by isolating cellular behavior from the complete architecture of thyroid follicles.
Their biology connects normal thyroid development and endocrine regulation with disease-related tissue changes. Studying these cells can help researchers examine how follicular organization and hormone-producing activity relate to goiter and thyroid cancer. Combining cellular, histological, and culture-based approaches also supports comparison between normal follicle function and altered tissue pathology.