Gene-based Techniques
Gene-based Techniques - Related Videos
05:33
Biolistic-Mediated Gene Transfer: A Technique to Deliver Gene of Interest in Target Cells via a Biolistic Gene Gun
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2025
In this video, we demonstrate the protocol of biolistic gene gun-mediated gene delivery in cultured cells. This method provides a simple method of stable genetic transformation using a minimal quantity of DNA.
02:30
A CRISPR-Cas9 Technique for Gene Editing in T Cells
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2025
This video demonstrates an assay for performing gene editing in human T cells using the CRISPR-Cas9 technology. A mixture of primary CD4+ and CD8+ T cells is combined with a CRISPR-Cas9 ribonucleoprotein complex, targeting specific genes for knockout. Upon electroporation, the sgRNA guides Cas9 to the target DNA sequence, creating precise cuts. These cuts are then repaired by the cell's non-homologous end-joining mechanism, leading to gene knockout.
07:27
CRISPR Interference-Based Gene Silencing: A Technique for Targeted Repression of Gene Function in Pathogenic Leptospira
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2025
This video demonstrates a CRISPR interference-based gene silencing in pathogenic Leptospira. Targeted repression of gene expression using a CRISPR/Cas system that blocks mRNA formation helps identify candidate genes for pathological and pharmacological studies.
03:31
Laser-Assisted Lentiviral Gene Delivery: A Technique to Permeabilize Mouse Fertilized Eggs to Facilitate Lentiviral Gene Delivery
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2025
In this video, we demonstrate the laser-assisted permeation of the protective layer of zona pellucida in mouse fertilized eggs for facilitating lentiviral gene delivery. Lentivirus enables the generation of transgenic animals with a gene of interest stably integrated into their genome.
05:29
3D DNA FISH: A Technique to Locate a Specific Gene on a Chromosome
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2023
Circulating tumor cells (CTC) isolation and characterization from the blood of cancer patients is a type of non-invasive liquid biopsy and associated with relapse and disease progression. This video describes the technique of DNA fluorescence-in-situ-hybridization (DNA FISH) on CTCs captured on a medical wire, to study a specific gene of interest.
FAQs
Gene-based techniques such as PCR amplify selected DNA sequences, increasing the amount of a target region available for analysis. This makes a chosen sequence easier to examine than the surrounding genetic material. In practice, amplification supports focused investigation of genetic regions, including work aimed at identifying mutations or distinguishing pathogen-associated sequences.
Sequencing determines nucleotide order, providing information that amplification alone cannot supply. The resulting sequence can reveal differences within a gene or other DNA region and therefore helps connect genetic variation with biological characteristics. In biology, this capability is valuable for studying mutations, inheritance, evolution, and disease-related genetic changes when sequence-level information is required.
Measuring gene expression from RNA shows which genetic instructions are active in a biological sample, rather than only what DNA sequence is present. This distinction helps researchers examine how cells use genetic information during development or disease. RNA-focused analysis therefore adds a functional layer to DNA studies and can help relate changes in expression to observed phenotypes.
CRISPR-Cas systems are useful when the research goal is to alter a chosen DNA region rather than merely observe it. Their targeted action enables functional studies in which researchers examine the consequences of changing a particular genetic site. This approach supports investigation of gene roles and the design of engineered cells, linking a deliberate DNA change to biological outcomes.
The choice follows the biological question. PCR suits goals requiring amplification of a selected sequence, while sequencing is appropriate when researchers need nucleotide order. RNA analysis addresses gene expression, and CRISPR-Cas serves studies that require alteration of a chosen DNA region. This division aligns each technique with the evidence or experimental change being sought.
Gene-based techniques connect genotype with phenotype and support the identification of mutations and pathogens. They also contribute to studies of development, disease, evolution, and biotechnology. Across biology, these methods can help describe genetic differences, examine how genetic information is expressed, or investigate the consequences of targeted DNA changes in cells and organisms.