Gene Insertion
Gene Insertion - Related Videos
04:24
TALEN-Mediated Targeted Gene Integration: Using Engineered Sequence-Specific Nucleases for the Precise Insertion of Fluorescent Protein Gene into hiPSCs
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2025
This video describes a precise genome editing technique in human induced pluripotent stem cells, or hiPSCs, using TALENs to create double-stranded breaks at a targeted locus, inducing homology-directed repair for fluorescence protein gene integration.
09:02
Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
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Cited by 4 •
2015
Gene targeting methodologies can be used to generate transgenic mice with knockout, knock-in and tagged alleles. Here, we describe an improved method of recombineering in E. coli, that we term ‘subcloning plus insertion’, which can be used to generate custom gene targeting vectors rapidly.
05:09
Binary Bacterial Artificial Chromosome Vector Based Gene Transformation in Arabidopsis Plants: A Technique to Generate Transgenic Plants With Single-Copy Insertion
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2025
In this video, we demonstrate generation of transgenic Arabidopsis plants expressing stable transgene through Agrobacterium-mediated binary bacterial artificial chromosome-based gene transformation.
11:39
Peripheral Intravenous Catheter Insertion
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2023
Source: Madeline Lassche, MSNEd, RN and Katie Baraki, MSN, RN, College of Nursing, University of Utah, UT The purpose of peripheral intravenous catheter (PIV) insertion is to infuse medications, perform intravenous (IV) fluid therapy, or inject radioactive tracers for special examination procedures. Placing a PIV is an invasive procedure and requires the use of an aseptic, no-touch technique. Common IV venipuncture sites are the arms and hands in adults and the feet in children. According to...
20:59
Large Insert Environmental Genomic Library Production
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Cited by 18 •
2009
Construction of a fosmid library with environmental genomic DNA isolated from the vertical depth continuum of a seasonally hypoxic fjord is described. The resulting clone library is picked into 384-well plates and archived for downstream sequencing and functional screening by the application of an automated colony picking system.
FAQs
These systems influence where donor DNA becomes integrated. Homologous recombination supports placement through matching DNA regions, whereas CRISPR-associated nucleases are engineered to act at a chosen genomic location. By directing the insertion rather than relying only on random placement, researchers can examine gene effects in a more controlled genetic context.
DNA repair converts the initial DNA-processing event into a stable genetic change. After donor DNA is delivered and the target sequence is acted upon, cellular repair mechanisms join or incorporate the introduced material into the genome or another genetic molecule. This stabilization allows the inserted sequence to persist for subsequent analysis or biological use.
Precision determines whether the added sequence reaches the intended site, while efficiency reflects how successfully the alteration occurs in target cells. Both properties affect how confidently researchers can connect an inserted gene with an observed effect. They also influence the practicality of applications ranging from functional genomics and recombinant protein production to emerging gene therapies.
A typical workflow begins by preparing donor DNA and delivering it into target cells. An enzyme-based or engineered insertion system then places the sequence at a chosen or random location, followed by cellular DNA repair. Researchers can subsequently examine whether the sequence became integrated and evaluate the resulting genetic or biological effects.
In functional genomics, researchers insert a sequence and study how the altered gene content changes cellular behavior, helping investigate gene function. The same strategy can support disease-mechanism studies by introducing genetic changes relevant to a condition and examining their effects. These experiments connect specific DNA alterations with biological outcomes.
Gene insertion supports the production of recombinant proteins by adding genetic instructions that cells can use to make a selected protein. It also contributes to the development of genetically modified organisms, in which altered gene content can be studied or applied in biotechnology. In biology, these uses extend gene insertion beyond basic mechanism studies.