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Large Insert Environmental Genomic Library Production

Large Insert Environmental Genomic Library Production

Article DOI: 10.3791/1387
September 23rd, 2009


Summary September 23rd, 2009

Construction of a fosmid library with environmental genomic DNA isolated from the vertical depth continuum of a seasonally hypoxic fjord is described. The resulting clone library is picked into 384-well plates and archived for downstream sequencing and functional screening by the application of an automated colony picking system.

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