Glucose Clearance
Glucose Clearance - Related Videos
05:52
In Vitro Glucose Depletion Assay: A Fluorescence-Based Method to Quantify Cellular Glucose Uptake via Extracellular Glucose Depletion Measurement
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2025
This video demonstrates an in vitro assay to indirectly measure intracellular glucose uptake. The assay uses a fluorescently-labeled glucose analog to establish a correlation between extracellular glucose depletion and intracellular glucose uptake.
02:23
Oral Glucose Tolerance Test (OGTT): Measuring Glucose Metabolism in Mice
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2023
This video introduces how to perform an oral glucose tolerance test on a laboratory mouse.
06:59
Characterization of Metabolic Status in Nonhuman Primates with the Intravenous Glucose Tolerance Test
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Cited by 7 •
2016
The goal of this protocol is to present a standard method to perform intravenous glucose tolerance tests (IVGTTs) to assess glycemic control in nonhuman primates and assess their metabolic status from healthy to dysmetabolic.
01:09
Hepatic Drug Clearance: Restrictive and Nonrestrictive Clearance
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2025
Hepatic clearance refers to the volume of blood cleared of a drug by the liver per unit of time. It plays a crucial role in drug metabolism and elimination. While hepatic clearance is commonly estimated by subtracting renal clearance from total body clearance, other pathways, such as pulmonary or biliary clearance, may also contribute. However, these pathways are generally less significant than hepatic and renal clearance. Most drugs undergo restrictive clearance, which is proportional to the...
04:11
Assessing Glucose Uptake in the Motor Neurons of Drosophila Larvae with Upregulated Glucose Metabolism
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2025
The video demonstrates a technique for measuring glucose uptake in Drosophila larval motor neurons using a fluorescence resonance energy transfer (FRET)-based intracellular glucose sensor. After dissecting the larvae to expose the ventral nerve cord, the motor neurons are visualized through the fluorescence emitted by the sensor's donor fluorophore. When stimulated with glucose, FRET between the sensor's donor and acceptor fluorophores triggers the emission of fluorescence from the acceptor,...
FAQs
Insulin increases glucose uptake by skeletal muscle and adipose tissue through GLUT4 transporters. This transport step moves circulating glucose into cells, where it can be used or stored, and therefore strongly affects how quickly blood glucose declines. Reduced responsiveness to insulin would be expected to limit this uptake and alter the interpretation of clearance measurements.
The liver helps regulate circulating glucose by storing it as glycogen after glucose enters the bloodstream. The kidneys contribute differently by reclaiming filtered glucose, but only up to their transport capacity. When that capacity is exceeded, glucose is no longer completely reclaimed, so renal handling becomes an important component of overall glucose regulation and clearance.
The measured rate can reflect several interacting processes: insulin-stimulated uptake by skeletal muscle and adipose tissue, hepatic glycogen storage, and the kidney’s ability to reclaim filtered glucose. Changes in insulin sensitivity, tissue glucose transport, liver storage, or renal transport capacity can therefore modify clearance and provide clues about altered metabolic regulation.
Glucose clearance measurements help characterize how effectively the body regulates glucose after it enters circulation. Faster or more efficient removal can provide information about insulin sensitivity and glucose tolerance, whereas altered clearance may indicate disrupted metabolic regulation. These measurements are therefore useful for examining glucose homeostasis rather than viewing blood glucose concentration in isolation.
In diabetes and obesity research, glucose clearance provides a measure for evaluating metabolic regulation and insulin-related glucose handling. Investigators can use it to characterize changes in glucose tolerance or insulin sensitivity associated with these conditions. The measure also supports comparisons of how disease-related metabolic changes affect the movement of glucose from blood into tissues.
Changes in glucose clearance can be used to assess whether a nutritional intervention or drug treatment affects glucose homeostasis. By examining how glucose removal changes after treatment, researchers can investigate effects on insulin sensitivity, tissue uptake, liver storage, or renal handling. This makes clearance a useful outcome for studying metabolic responses to interventions.