Hematological Analysis
Hematological Analysis - Related Videos
11:08
Endothelialized Microfluidics for Studying Microvascular Interactions in Hematologic Diseases
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Cited by 52 •
2012
A method to culture an endothelial cell monolayer throughout the entire inner 3D surface of a microfluidic device with microvascular-sized channels (<30 μm) is described. This in vitro microvasculature model enables the study of biophysical interactions between blood cells, endothelial cells, and soluble factors in hematologic diseases.
08:29
Biomarkers in an Animal Model for Revealing Neural, Hematologic, and Behavioral Correlates of PTSD
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Cited by 29 •
2012
We describe a rat model of post traumatic stress disorder (PTSD) that reveals the persistent alterations in neuroendocrine function and the delayed long-term, exaggerated fear response, characteristic of PTSD patients. The animal model and methods described here are useful for correlating biomarkers in brain nuclei, which are mechanistic but cannot be measured in patients, with biomarkers in peripheral white blood cells, which can.
02:34
Histological and Hematological Analyses to Evaluate the Effects of Bacillus subtilis in Heat-Stressed Rats
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2025
Source: Sorokulova, I., et al. Prevention of Heat Stress Adverse Effects in Rats by Bacillus subtilis Strain. J. Vis. Exp. (2016)This video presents histological and blood microscopy analyses used to evaluate the protective effects of Bacillus subtilis in rats exposed to heat stress.
08:46
A Real-time Potency Assay for Chimeric Antigen Receptor T Cells Targeting Solid and Hematological Cancer Cells
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Cited by 10 •
2019
We describe a quantitative real-time in vitro cytolysis assay system to evaluate the potency of chimeric antigen receptor T cells targeting liquid and solid tumor cells. This protocol can be extended to assess other immune effector cells, as well as combination treatments.
09:05
Methylation Specific Multiplex Droplet PCR using Polymer Droplet Generator Device for Hematological Diagnostics
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2020
Epigenetic markers are used for white blood cell (WBC) subtyping through the quantification of DNA methylation patterns. This protocol presents a multiplex droplet polymerase chain reaction (mdPCR) method using a thermoplastic elastomer (TPE)-based microfluidic device for droplet generation allowing for precise and multiplex methylation-specific target quantification of WBC differential counts.
FAQs
Cell counts provide quantitative measurements of red blood cells, white blood cells, and platelets, while morphology examines their physical characteristics. Used together, these approaches offer both numerical and structural information about blood. This combination helps investigators relate changes in cell abundance or appearance to oxygen transport, immune activity, coagulation, inflammation, or broader physiological disturbances.
Anticoagulated blood helps preserve a sample in a condition suitable for particular assays by limiting unwanted clot formation. This preparation supports measurements involving circulating blood cells and allows the sample to be separated or processed according to the test. The choice to use anticoagulated material therefore depends on the components and properties the analysis is intended to examine.
Cell counts and morphology describe cellular quantity and appearance, whereas biochemical and immunological tests provide additional information about blood or plasma composition and immune-related activity. Combining these measurement types broadens interpretation beyond cell structure alone. It can help characterize physiological function and investigate processes associated with inflammation, immune-system disorders, or other changes affecting blood.
Different measured blood components correspond to different physiological roles. Red blood cell findings relate to oxygen transport, white blood cell findings reflect immune activity, and platelet findings inform coagulation. Plasma-based biochemical or immunological measurements add further context, including inflammation-related changes. Comparing these results helps organize observations according to the biological function most affected.
A workflow begins by obtaining blood and selecting preparation conditions appropriate to the intended assay. When required, anticoagulated blood is used, then the sample may be separated or otherwise prepared for specific measurements. Analysts can subsequently combine cell counting, morphology, and biochemical or immunological testing to generate complementary information about cells, plasma, and physiological status.
Hematological analysis is useful when researchers need to study blood-related physiology, disease, or treatment effects. In biology, it supports investigations of development and homeostasis, meaning the maintenance of stable internal conditions. In clinical and translational settings, the same measurements assist diagnosis and treatment monitoring, while also supporting research on disorders involving blood or the immune system.