Hemoglobin Measurement
Hemoglobin Measurement - Related Videos
01:24
Hemoglobin
0 Views •
2024
Hemoglobin is a globular protein made up of four subunits. Two of these subunits are alpha chains, and the other two are beta chains. Each subunit contains a molecule of heme, which has an iron atom and can bind to oxygen. When an oxygen molecule binds to one heme group, it changes the shape of hemoglobin, making it easier for the other heme groups to bind oxygen as well. When all four heme groups are bound to oxygen, the resulting molecule is called oxyhemoglobin. As a result, arterial blood...
06:37
Staphylococcus aureus Growth using Human Hemoglobin as an Iron Source
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Cited by 31 •
2013
Here we describe a growth assay for Staphylococcus aureus using hemoglobin as the sole source of available nutrient iron. This assay establishes the role of bacterial factors involved in hemoglobin-derived iron acquisition.
05:18
A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering
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Cited by 1 •
2016
A photo-thermal angular light scattering (PT-AS) sensor enables the rapid and chemical-free hemoglobin assay of nanoliter-scale blood samples. Here, details of the PT-AS setup and a measurement protocol for the hemoglobin concentration in blood are provided. Representative results for anemic blood samples are also presented.
04:51
Modeling Neonatal Intraventricular Hemorrhage Through Intraventricular Injection of Hemoglobin
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2025
This video demonstrates a method to generate a rat model of neonatal intraventricular hemorrhage. In this procedure, an anesthetized rat pup is injected with hemoglobin into the lateral ventricle of the brain. The hemoglobin causes oxidative stress and releases heme, simulating intraventricular hemorrhage. The resulting damage to brain tissues, driven by reactive oxygen species and inflammatory cytokines, leads to ventricular enlargement, a common consequence of intraventricular hemorrhage.
07:57
Modeling Neonatal Intraventricular Hemorrhage Through Intraventricular Injection of Hemoglobin
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Cited by 12 •
2022
We present a model of neonatal intraventricular hemorrhage using rat pups that mimics the pathology seen in humans.
FAQs
In a common photometric workflow, red blood cells are first lysed so their hemoglobin is released into the sample. The released hemoglobin is then converted into a stable colored derivative. A photometric instrument measures the resulting light absorbance, creating a signal that can be related to hemoglobin concentration through calibration standards. This sequence connects sample preparation with quantitative analysis.
Calibration standards provide reference points for interpreting the sample’s absorbance. The instrument compares the light absorbed by the processed blood sample with the absorbance associated with those standards, allowing the result to be expressed as a hemoglobin concentration rather than as an uncalibrated optical signal. This comparison supports consistent measurement across samples and experimental or patient populations.
Automated analyzers can incorporate the hemoglobin result into a complete blood count rather than treating it as an isolated measurement. This integration places the concentration alongside other hematological data collected by the analyzer, making the result useful for broader blood-profile assessment. It also supports standardized comparisons when researchers or clinicians evaluate multiple samples.
A common setup requires a blood sample, a process that lyses red blood cells, a means of converting hemoglobin into a stable colored derivative, calibration standards, and a photometric instrument. The workflow proceeds from sample preparation to color development and absorbance measurement. An automated analyzer may combine these functions within a complete blood count.
Hemoglobin measurement supports biological studies of respiration and circulation because hemoglobin concentration relates to blood oxygen-carrying capacity. It can also contribute to research on nutrition and disease progression. By providing a measurable blood variable, the assay helps researchers compare physiological or disease-related patterns across experimental groups and patient populations.
Measured hemoglobin concentration can help identify anemia, polycythemia, and blood loss, making it relevant to assessment of hematological health. The result can also guide treatment decisions and support comparisons among experimental or patient populations. Interpreting it within these contexts connects a numerical assay result with disease status, physiological function, or changes over time.