Tropic Response Screening
Tropic Response Screening - Related Videos
12:18
A Strategy to Validate the Role of Callose-mediated Plasmodesmal Gating in the Tropic Response
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Cited by 7 •
2016
This article describes the methods for screening the genes controlling plasmodesmal permeability and hence auxin gradient during tropic response. This includes the measurement of the degree of tropic response in hypocotyl of Arabidopsis thaliana and checking plasmodesmal permeability by 8-hydroxypyrene-1,3,6-trisulfonic acid (HPTS) loading and finally callose level assessment.
10:25
Competitive Homing Assays to Study Gut-tropic T Cell Migration
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Cited by 11 •
2011
Competitive homing experiments allow to directly assessing the migratory properties of two different cell populations in a single mouse. Here we illustrate this procedure by comparing the migration of ex vivo-generated gut-tropic versus non-gut tropic T cells.
12:50
Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response
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Cited by 1 •
2017
Vascular endothelium tightly controls leukocyte recruitment. Inadequate leukocyte extravasation contributes to human inflammatory diseases. Therefore, searching for novel regulatory elements of endothelial activation is necessary to design improved therapies for inflammatory disorders. Here, we describe a comprehensive methodology to characterize novel endothelial regulators that can modify leukocyte trafficking during inflammation.
06:25
Evaluating Toxicity of Chemicals using a Zebrafish Vibration Startle Response Screening System
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Cited by 6 •
2024
We describe a screening system's workflow and data analysis for evaluating chemical compound toxicity based on the zebrafish embryo vibration startle response. The system records the movements of zebrafish embryos upon exposure to a vibration stimulus and allows for an integrated evaluation of general toxicity/lethality and neuromuscular toxicity.
08:50
Genetic Screens
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2023
Genetic screens are critical tools for defining gene function and understanding gene interactions. Screens typically involve mutating genes and then assessing the affected organisms for phenotypes of interest. The process can be “forward”, where mutations are generated randomly to identify unknown genes responsible for the phenotypes, or it can be “reverse”, where specific genes are targeted for mutation to observe what phenotypes are produced.Here, JoVE reviews various types of genetic...
FAQs
A directional stimulus is detected across a plant tissue rather than at only one isolated point. Signaling then redistributes auxin, creating unequal concentrations on different sides of a root or shoot. Because auxin affects cell elongation unevenly, one side grows differently from the other, producing measurable curvature that reveals the tissue’s response.
They are distinguished by the environmental cue used to direct growth: light for phototropism, gravity for gravitropism, and water for hydrotropism. The underlying screening logic remains comparable because each test examines directional growth, but the stimulus-specific signaling response can reveal different genes or pathways involved in environmental sensing and plant development.
Growth angle, curvature, and response timing provide complementary measurements. Angle or curvature describes the magnitude and direction of bending, while timing indicates how quickly a response develops. Comparing these values across genotypes or treatment conditions can separate differences in response strength from differences in response speed, helping identify biological factors that influence directional growth.
The central comparisons are plant genotype and treatment condition, evaluated under a defined directional stimulus. Consistent scoring of growth angle, curvature, or response timing makes differences easier to attribute to genetic or treatment effects. These comparisons can indicate whether a gene, signaling pathway, or environmental condition changes the strength or timing of tropic growth.
When genotypes show different bending angles, curvature, or response timing under the same stimulus, those differences provide clues about genetic control of tropic behavior. Screening can therefore help identify genes and pathways associated with phototropism, gravitropism, or hydrotropism. The resulting comparisons connect observable plant growth with the mechanisms that regulate environmental responses.
Directional growth responses influence how plants adjust to environmental conditions, making them relevant to both development and adaptation. By comparing responses among genotypes or treatments, researchers can investigate traits linked to environmental sensing and growth regulation. This information supports studies of plant development and may help evaluate characteristics of interest for crop improvement.