Hyphae

Hyphae are threadlike cellular structures that form the basic growth units of most fungi, enabling these organisms to expand through soil, plant tissue, food, and other substrates. Their tips grow by polarized extension: vesicles deliver membrane and cell-wall materials to the apex, while branching and, in many species, cross-walls called septa organize the filament and maintain cytoplasmic continuity. Collectively, hyphae form a mycelium that secretes extracellular enzymes, breaks down complex materials, and absorbs released nutrients. Studying hyphal growth helps explain fungal decomposition, plant symbioses, and disease, while informing research on antibiotics, industrial enzymes, biomaterials, and fungal ecology.

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Research

JoVE EoE - Immunodiagnostics

A Method for Sample Preparation to Visualize Biofilm Forming Bacteria on Fungal Hyphae

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2025

This video describes a method to grow and qualitatively analyze bacterial biofilms on fungal hyphae using microscopy. Bacterial biofilms frequently form on fungal surfaces and involve numerous bacterial-fungal interaction processes, such as metabolic cooperation, competition, or predation.

A Technique to Quantify Fungal Infection in Zebrafish Larvae

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2025

This video demonstrates a method to quantify fungal infection in zebrafish larvae. Fungal spores are microinjected into the larval hindbrain ventricle, and the larvae are incubated to allow infection development. Infected larvae are harvested at defined intervals, and infection progress is assessed by enumerating colony-forming units.

Establishment of Rice Plant Leaf Infection through Fungal Inoculation via Wounding

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2025

This video demonstrates the establishment of rice plant leaf infection through wounding inoculation with Magnaporthe grisea mycelium. The fungal spore-derived germ tube penetrates the epidermal layer, resulting in tissue infection and lesions on the leaves. This method aids pathotype screening and improves plant resistance understanding for molecular breeding.

Education

JoVE Science Education - Environmental Sciences

Filamentous Fungi

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2023

Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba - The University of Arizona Demonstrating Author: Bradley Schmitz Fungi are heterotrophic eukaryotic organisms, and with the exception of yeasts, are aerobic. They are abundant in surface soils and are important for their role in nutrient cycling and the decomposition of organic matter and organic contaminants. White rot fungi (phanerochaete chryosporium) for example, (Figure 1) are known to degrade aromatics. Figure 1. White rot on...

Fluorescence Time-Lapse Imaging of the Streptomyces venezuelae Life Cycle

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2025

Source: Schlimpert, S. et al. Fluorescence Time-lapse Imaging of the Complete S. venezuelae Life Cycle Using a Microfluidic Device. J. Vis. Exp. (2016)This video demonstrates fluorescence time-lapse imaging of the complete life cycle of the bacteria Streptomyces venezuelae using a microfluidic device to visualize germination, hyphal growth, and sporulation in real time.

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