Hypoxia Incubator
Hypoxia Incubator - Related Videos
07:01
Induction and Testing of Hypoxia in Cell Culture
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Cited by 247 •
2011
Here we propose simple methods to induce hypoxia in cell cultures and simple tests to evaluate the hypoxic status of the cultures.
08:01
Induction of Hypoxia in Living Frog and Zebrafish Embryos
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Cited by 5 •
2017
We introduce a novel hypoxic chamber system for use with aquatic organisms such as frog and zebrafish embryos. Our system is simple, robust, cost-effective and allows the induction and sustainment of hypoxia in vivo and for up to 48 h. We present 2 reproducible methods to monitor the effectiveness of hypoxia.
02:54
Hypoxia-Induced Climbing Behavior of Drosophila melanogaster
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2026
Source:Siddarth Gunnala1, Nafisa M. Jadavji2,3,41Department of Biomedical Sciences, Midwestern University, Glendale, AZ, USA2Division of Integrative and Molecular Physiology, Department of Biomedical Sciences, Southern University of Illinois, Carbondale, IL, USA3Department of Child Health, College of Medicine Phoenix, University of Arizona, Phoenix, AZ, USA4Department of Neuroscience, Carleton University, Ottawa, ON, CanadaThis video demonstrates hypoxia-induced climbing behavior in Drosophila...
13:13
Biochemical Measurement of Neonatal Hypoxia
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Cited by 19 •
2011
A method is described to measure biochemical markers of neonatal hypoxia-ischemia. The approach utilizes high pressure liquid chromatography (HPLC) and Gas Chromatography Mass Spectrometry (GC/MS).
12:03
In Vivo Model for Testing Effect of Hypoxia on Tumor Metastasis
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Cited by 6 •
2016
This manuscript describes the development of an animal model that allows for the direct testing of the effects of tumor hypoxia on metastasis and the deciphering the mechanisms of its action. Although the experiments described here focus on Ewing sarcoma, a similar approach can be applied to other tumor types.
FAQs
The device lowers oxygen by replacing or diluting ambient air with a regulated gas mixture, commonly using nitrogen, while separately controlling carbon dioxide, temperature, and humidity. Sensors measure chamber conditions, and feedback systems adjust gas delivery to maintain selected set points. This stability lets cultures experience defined oxygen exposure rather than uncontrolled environmental fluctuations.
A hypoxia experiment does not change oxygen in isolation. Controlling carbon dioxide, temperature, and humidity helps maintain the intended culture environment while oxygen is reduced. Coordinated regulation makes it easier to attribute observed cellular responses to the selected oxygen condition and supports consistent comparisons among cultures exposed to different experimental settings.
Sensors continuously monitor the incubator environment, while feedback systems use those measurements to help preserve the chosen conditions. This regulation is important because the experiment depends on maintaining a defined oxygen concentration together with controlled carbon dioxide, temperature, and humidity. Reliable monitoring therefore supports reproducible studies of oxygen-dependent cellular behavior.
Culturing cells under controlled low-oxygen conditions allows researchers to examine responses mediated by hypoxia-inducible factors, or HIFs. These experiments can connect a defined oxygen environment with changes in cellular adaptation, metabolism, and gene regulation. The approach is therefore useful for studying how oxygen availability influences biological responses in controlled culture models.
A basic setup requires selecting the target oxygen concentration and defining the accompanying carbon dioxide, temperature, and humidity conditions. Researchers then use an appropriate regulated gas mixture, often involving nitrogen to reduce oxygen, and rely on the incubator's sensors and feedback controls. Establishing these parameters creates a consistent environment for comparing biological responses across cultures.
Researchers use this system when they need to model oxygen-limited environments associated with tumors, developing tissues, or ischemic injury. The resulting cultures can support investigations of cellular adaptation, metabolism, gene regulation, and HIF-mediated responses. Hypoxia incubators also contribute to disease modeling, drug testing, and cell-based research where controlled oxygen exposure is scientifically relevant.