Endothelial Calcium
Endothelial Calcium - Related Videos
10:46
Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells
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Cited by 25 •
2013
Intercellular Ca2+-waves are driven by gap junction channels and hemichannels. Here, we describe a method to measure intercellular Ca2+-waves in cell monolayers in response to a local single-cell mechanical stimulus and its application to investigate the properties and regulation of gap junction channels and hemichannels.
12:43
On-Chip Endothelial Inflammatory Phenotyping
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Cited by 3 •
2012
Microfluidic flow chambers etched by photolithography and fabricated from PDMS are applied to probe functional outcomes associated with EC dysfunction and inflammation. In a representative experiment, the ability of differential shear stress to modulate monocytic cell adhesion to cytokine activated EC monolayers is demonstrated.
02:38
Analysis of Calcium Dynamics and Membrane Potential Changes in a Mouse Arteriolar Endothelium
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2025
Source: Hakim, M. A. et al., Isolation and Functional Analysis of Arteriolar Endothelium of Mouse Brain Parenchyma. J. Vis. Exp. (2022).This video demonstrates the functional analysis of an arteriolar endothelial tube isolated from a mouse brain. It outlines the steps for measuring intracellular calcium levels and membrane potential, both at baseline and in response to pharmacological stimulation.
09:23
Isolation of Microvascular Endothelial Tubes from Mouse Resistance Arteries
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Cited by 20 •
2013
We present a preparation for visualizing and manipulating calcium signaling in native, intact microvascular endothelium. Endothelial tubes freshly isolated from mouse resistance arteries supplying skeletal muscle retain in vivo morphology and dynamic signaling within and between neighboring cells. Endothelial tubes can be prepared from microvessels of other tissues and organs.
16:33
Functional Calcium Imaging in Developing Cortical Networks
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Cited by 32 •
2011
Spontaneous activity of developing neuronal networks can be measured using AM-ester forms of calcium-sensitive indicator dyes. Changes in intracellular calcium, indicating neuronal activation, are detected as transient changes in indicator fluorescence with one- or two-photon imaging. This protocol can be adapted for a range of developmentally-dependent neuronal networks in vitro.
FAQs
Calcium signals can arise from two coordinated sources: ions entering through the plasma membrane and ions released from intracellular stores. The resulting change in cytosolic calcium activates calcium-sensitive proteins, allowing endothelial cells to convert an ionic signal into functional responses. This organization helps connect changes in the cellular environment with regulation of vessel behavior.
Calmodulin serves as a calcium-sensitive signaling partner that helps transmit changes in cytosolic calcium to downstream proteins. In endothelial cells, this signaling includes activation of endothelial nitric oxide synthase. The connection is important because it links calcium dynamics to nitric oxide production, which contributes to vascular relaxation and therefore influences blood vessel function.
Calcium waves allow signaling changes to spread between neighboring endothelial cells rather than remaining confined to one cell. This intercellular communication can coordinate vascular responses across a larger endothelial region. Studying these waves helps researchers examine how local calcium events may influence broader processes, including barrier permeability, inflammation, and coordinated blood vessel regulation.
Researchers measure endothelial calcium dynamics by tracking changes in intracellular calcium over time. These measurements can be related to processes such as vascular tone, angiogenesis, barrier permeability, and inflammatory signaling. The approach is useful because it examines signaling behavior directly, helping investigators assess how endothelial cells respond during normal vascular function or dysfunction.
Endothelial calcium research supports investigations of vascular tone and angiogenesis, the formation and development of blood vessels. It also helps characterize endothelial dysfunction associated with cardiovascular disease. By examining calcium-linked signaling and its effects on vessel behavior, researchers can evaluate biological mechanisms and investigate potential therapeutic approaches relevant to vascular health.
Changes in endothelial calcium signaling can be examined alongside outcomes such as nitric oxide production, vascular relaxation, barrier permeability, and inflammation. This provides a way to study how altered endothelial behavior may contribute to cardiovascular disease. Measurements can also support evaluation of potential therapies by showing whether calcium-related vascular signaling is modified.