In Vitro Expansion Culture
In Vitro Expansion Culture - Related Videos
10:15
Isolation and Culture Expansion of Tumor-specific Endothelial Cells
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Cited by 20 •
2015
We report a reliable method to isolate and culture primary tumor-specific endothelial cells from genetically engineered mouse models.
08:03
An In Vitro Organ Culture Model of the Murine Intervertebral Disc
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Cited by 22 •
2017
Whole organ culture of the intervertebral disc (IVD) preserves the native extracellular matrix, cell phenotypes, and cellular-matrix interactions. Here we describe an IVD culture system using mouse lumbar and caudal IVDs in their functional spinal units and several applications utilizing this system.
08:39
Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro
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Cited by 27 •
2010
T lymphocyte migration occurs during homing to lymphoid organs, exit from the vasculature, and entering into peripheral tissues. Here, we describe a protocol that can be used to analyze T lymphocyte migration in vitro.
09:42
Analysis of Thermal Expansion via Dilatometry
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2023
Source: J. Jacob Chavez, Ryan T. Davis, and Taylor D. Sparks, Department of Materials Science and Engineering, The University of Utah, Salt Lake City, UT Thermal expansion is extremely important when considering which materials will be used in systems that experience fluctuations in temperature. A high or low thermal expansion in a material may or may not be desirable, depending on the application. For instance, in a common liquid thermometer, a material with a high thermal expansion would be...
03:05
In Vitro Culture and Differentiation of Primary Monocytes into Macrophages
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2025
This video demonstrates the priming of primary monocytes into macrophages. Primary human monocytes can be isolated and differentiated to an M1-like pro-inflammatory phenotype using the granulocyte-macrophage colony-stimulating factor (GM-CSF) or to an M2-like anti-inflammatory phenotype using the macrophage colony-stimulating factor (M-CSF).
FAQs
These conditions determine whether cells remain viable and continue proliferating during culture. Nutrient media supplies the chemical environment, while suitable temperature, pH, and gas levels help maintain cellular function. If conditions are poorly matched to the cells, expansion may become less reliable, reducing the consistency of material available for biological experiments.
Growth factors are included when cells require additional signals to support proliferation or maintain a desired biological state. Their use is therefore cell-dependent rather than universal. Selecting suitable growth-factor conditions can help researchers generate sufficient populations while preserving properties relevant to studies of cell function, differentiation, disease mechanisms, or experimental treatment responses.
Passaging transfers proliferating cells into fresh vessels containing suitable nutrient media. This repeated renewal provides space and refreshed culture conditions that allow expansion to continue rather than ending in the original vessel. The procedure also helps researchers generate larger populations for experiments that require more biological material or more consistent cell numbers.
Controlled expansion produces larger cell populations under defined environmental conditions, which can reduce variation in the material used between experiments. More consistent populations help researchers compare cell behavior, differentiation, disease-related mechanisms, and treatment responses with greater confidence. The value comes from linking cell production to controlled media, culture conditions, and scheduled transfer into fresh vessels.
A typical workflow begins by placing cells in sterile nutrient media and establishing suitable temperature, pH, and gas conditions. Researchers maintain the cultures while the cells proliferate, then transfer them into fresh vessels as needed. Repeating this cycle sustains expansion and generates the quantity of biological material required for downstream research.
Researchers use expansion culture when experiments require more cells than are initially available or need a relatively consistent population across repeated tests. The resulting material can support investigations of cell function, differentiation, disease mechanisms, and responses to experimental treatments. It is also relevant when biological studies require cells to be maintained outside the organism under controlled conditions.
By increasing cell numbers under controlled laboratory conditions, the method can supply biological material for tissue engineering and regenerative biology. Expanded populations may support research that requires reproducible starting material before examining cell behavior or developing tissue-related approaches. Its contribution lies in producing sufficient, more consistent cells for studies beyond basic cell maintenance.