In Vivo Transplantation
In Vivo Transplantation - Related Videos
08:12
Normothermic Ex Vivo Kidney Perfusion for the Preservation of Kidney Grafts prior to Transplantation
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Cited by 43 •
2015
The severe organ shortage has resulted in increased use of marginal kidney grafts for transplantation. This has triggered interest in alternative storage methods, since marginal grafts especially tolerate cold storage poorly. The technique of normothermic ex vivo kidney perfusion (NEVKP) represents a novel preservation method for kidney grafts prior to transplantation.
18:48
In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients
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Cited by 1 •
2017
Here, we present a protocol to induce tolerance in transplantation, and assess in vitro and in vivo the suppressive capacity of distinct cell subsets from the recipient and the immune status of the recipient toward donor or exogenous antigens.
09:21
Viral-mediated Labeling and Transplantation of Medial Ganglionic Eminence (MGE) Cells for In Vivo Studies
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Cited by 35 •
2015
GABAergic cortical interneuron progenitors disperse, develop and synaptically integrate into a host cortex after transplantation. These cells can be easily transduced before transplantation for in vivo studies of genetically modified GABAergic precursors. Here, we show viral labeling techniques to target specific interneuron subgroups using existing Cre lines and Cre-dependent reporters.
11:39
Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos
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Cited by 4 •
2016
Combining cell transplantation, cytoskeletal labeling and loss/gain of function approaches, this protocol describes how the migrating zebrafish prospective prechordal plate can be used to analyze the function of a candidate gene in in vivo cell migration.
05:49
Establishment of an Ex Vivo Lung Perfusion Rat Model for Translational Insights in Lung Transplantation
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Cited by 5 •
2023
Here, we describe the necessary steps for establishing a rat EVLP model and show the inflammatory profile associated with the perfused lungs. The aim is to propagate knowledge and experiences about the rat EVLP model, enabling the integral understanding of the biological responses associated with that revolutionary technique.
FAQs
A graft must interact with the host environment and obtain adequate nutrient and blood supply after implantation. Without these conditions, cells or tissues may not remain viable or function as intended. Assessing these requirements helps researchers interpret graft survival, integration, and therapeutic potential in vivo.
Immune recognition can identify the graft as different from the recipient’s own tissues and trigger rejection. This host response is therefore a major factor when evaluating whether transplanted cells, tissues, or organs persist and function. Studying it helps researchers understand host-graft interactions and determine whether immune recognition must be managed.
The host environment provides a physiologically relevant setting in which a graft encounters living biological conditions rather than being evaluated in isolation. This context allows researchers to examine how the transplanted material survives, integrates with the recipient, and produces therapeutic effects, providing information about responses that may be difficult to assess outside a living system.
Graft survival concerns whether the transplanted material remains viable after implantation, whereas integration concerns how it interacts with and becomes established within the host environment. Therapeutic effect is a further outcome, reflecting whether the graft produces a beneficial functional change. Separating these outcomes helps researchers interpret transplantation results more precisely.
Researchers assess whether the graft survives, establishes adequate interaction with the host, integrates into the recipient environment, and produces a therapeutic effect. They may also examine biological responses associated with immune recognition and rejection. Together, these outcomes show whether the transplant is functioning as intended and reveal limitations that could affect its potential use.
In vivo transplantation supports studies of tissue regeneration, stem cell behavior, organ function, and disease progression. By placing the graft in a living recipient, researchers can evaluate therapeutic potential alongside survival, integration, and host responses. These findings can guide regenerative treatment development and improve understanding of how transplanted material behaves biologically.