Injection Microscope
Injection Microscope - Related Videos
07:00
Intra-iliac Artery Injection for Efficient and Selective Modeling of Microscopic Bone Metastasis
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Cited by 43 •
2016
This manuscript provides the detailed procedure of intra-iliac artery (IIA) injection, a technique to deliver cancer cells specifically to hind limb tissues including bones to establish experimental bone metastases. Although initially established with breast tumor models, this protocol can be easily extended to other cancer types.
04:57
Proper Care and Cleaning of the Microscope
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Cited by 2 •
2008
Keeping the microscope optics clean is important for high-quality imaging. Dust, fingerprints, excess immersion oil, or mounting medium on or in a microscope causes reduction in contrast and resolution. DIC is especially sensitive to contamination and scratches on the lens surfaces. This protocol details the procedure for keeping the microscope clean.
08:08
Major Components of the Light Microscope
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2008
The light microscope is a basic tool for the cell biologist, who should have a thorough understanding of how it works, how it should be aligned for different applications, and how it should be maintained as required to obtain maximum image-forming capacity and resolution. The components of the microscope are described in detail here.
08:28
Zebrafish Brain Ventricle Injection
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Cited by 73 •
2009
After neural tube formation, the neuroepithelium constricts and folds while the tube fills with embryonic cerebrospinal fluid (eCSF) to form the embryonic brain ventricles. We developed this ventricle injection technique to better visualize the fluid filled space in contrast to the neuroepithelial shape in a live embryo.
03:50
Microscopic Analysis of Bacterial Growth Under Stress Using Microfluidic Chambers
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2025
Source: Cayron, J., et al. Multi-scale Analysis of Bacterial Growth Under Stress Treatments. J. Vis. Exp. (2019).This video demonstrates how to prepare and use a microfluidic plate for imaging bacterial cells under stress conditions. It outlines the process of introducing a bacterial culture and a stress-inducing antibiotic medium, followed by cell loading and microscopic setup. Time-lapse imaging in phase-contrast mode is then used to observe the development of elongated, filamentous cells...
FAQs
The micromanipulator holds the fine injection pipette and allows the operator to position it and control its movement with precision. Because the pipette is moved while the specimen remains visible through the microscope, the operator can direct delivery toward a selected cell, embryo, or tissue region rather than manipulating the specimen without visual control.
Visual guidance links the pipette’s movement to the specimen’s exact location. This helps the operator target a particular cell, embryo, or tissue area and observe the biological material as it is delivered. The combined view and manipulation are important when experiments require targeted introduction rather than general exposure of the specimen.
The system can deliver fluids, particles, and biological material, including genetic material, reagents, or tracers. The selected material depends on the biological question, such as introducing genetic information, applying a reagent, or marking a structure for observation. This range allows the same microscope-based approach to support different kinds of cellular and developmental experiments.
A basic workflow begins by placing the specimen within the microscope’s field of view and securing the injection pipette in the micromanipulator. The operator then positions the pipette under magnified observation, directs it toward the chosen target, delivers the selected material, and monitors the specimen for the resulting response. The procedure connects each manipulation step with immediate visual feedback.
Injection microscopy can be applied to cells, embryos, and tissues. These targets allow researchers to examine biological effects at different levels, from cellular responses to developmental changes. The appropriate target depends on the experimental objective, while the microscope and micromanipulator provide the visual access and positional control needed for localized delivery.
Researchers would choose this approach when they need to introduce a material into a defined biological target and then study the target’s response. It is particularly relevant for experiments involving genetic material, reagents, or tracers, because delivery and observation occur in the same microscope-guided setting. This supports investigations of cellular function, development, and experimental responses.
After delivery, researchers can examine observable biological outcomes associated with the introduced material. Depending on the experiment, these outcomes may involve cellular function, developmental processes, or responses to a reagent or tracer. The method is valuable because it ties a deliberately targeted intervention to subsequent observations in the manipulated cell, embryo, or tissue.