Intestinal Epithelial Cells
Intestinal Epithelial Cells - Related Videos
02:28
Assessing Transcytosis of E. coli Across Intestinal Epithelial Monolayers
0 Views •
2025
Source: Islam, A., et al., Assessment of Intestinal Transcytosis of Neonatal Escherichia coli Bacteremia Isolates. J. Vis. Exp. (2023)This video demonstrates the procedure for assessing transcytosis of neonatal Escherichia coli across intestinal epithelial monolayers using transwell inserts, electrical resistance measurement, and bacterial quantification.
10:52
Enteric Bacterial Invasion Of Intestinal Epithelial Cells In Vitro Is Dramatically Enhanced Using a Vertical Diffusion Chamber Model
0 Views •
Cited by 4 •
2013
Performing cell culture assays for investigating bacterial adhesion and invasion under aerobic conditions is usually unrepresentative of the in vivo environment. A Vertical Diffusion Chamber model allows study of interactions of the human pathogen Campylobacter jejuni with intestinal epithelial cells under more in vivo-like conditions, resulting in enhanced bacterial invasion.
11:27
Methods to Study Epithelial Transport Protein Function and Expression in Native Intestine and Caco-2 Cells Grown in 3D
0 Views •
Cited by 16 •
2017
We describe simple methods to study the regulation of intestinal serotonin transporter (SERT) function and expression using an in vitro cell culture model of Caco-2 cells grown in 3D and an ex vivo model of mouse intestines. These methods are applicable to the study of other epithelial transporters.
09:39
Determination of Tolerable Fatty Acids and Cholera Toxin Concentrations Using Human Intestinal Epithelial Cells and BALB/c Mouse Macrophages
0 Views •
2013
We set out to determine tolerable concentrations of three fatty acids (oleic, linoleic and linolenic acids) and cholera toxin that did not significantly and adversely affect cell survival by solubilizing the fatty acids and the toxin and using them in cell survival assays.
08:22
Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells
0 Views •
Cited by 8 •
2022
This protocol offers detailed instructions for establishing murine small intestine organoids, isolating type-1 innate lymphoid cells from the murine small intestine lamina propria, and establishing 3-dimensional (3D) co-cultures between both cell types to study bi-directional interactions between intestinal epithelial cells and type-1 innate lymphoid cells.
FAQs
Tight junctions determine how effectively material passes between adjacent intestinal epithelial cells. By regulating this paracellular route, they help maintain selective permeability rather than allowing uncontrolled movement from gut contents into underlying tissue. This control is central to barrier integrity and provides a mechanistic link between epithelial organization and conditions in which barrier disruption contributes to inflammation.
Different intestinal epithelial cell types divide labor within the same tissue. This organization allows the lining to coordinate nutrient absorption, secretion, and communication with underlying tissues instead of performing every function through one uniform population. Studying this cellular diversity helps researchers determine how changes in one epithelial activity may influence the broader digestive and barrier interface.
Continuous renewal from intestinal stem cells replaces epithelial cells exposed to food, microbes, and digestive chemicals. This ongoing replenishment supports tissue integrity despite the demanding conditions at the gut surface. The renewal process is therefore important for understanding how the epithelium remains functional over time and how impaired maintenance could contribute to barrier disruption.
These gut contents create persistent environmental challenges for intestinal epithelial cells. The tissue must preserve selective permeability while remaining in contact with substances that could disturb its organization or function. Studying this interface helps explain why epithelial integrity is closely connected to inflammation, barrier disruption, and interactions between host tissues and resident microbes.
Organoid models provide a research context for examining intestinal epithelial organization and function under controlled experimental conditions. They can support studies of barrier behavior, epithelial renewal, and interactions relevant to disease. Because the source material links these models with therapeutic development, organoids also help connect biological observations to the evaluation of potential treatments.
Their barrier, absorptive, secretory, and communication functions make intestinal epithelial cells central to studies of inflammatory bowel disease. When epithelial organization or permeability is disrupted, interactions between gut contents and underlying tissues may change, contributing to inflammatory processes. Examining these cells can therefore clarify disease mechanisms and identify biological processes relevant to therapeutic development.
Intestinal epithelial cells form the interface through which underlying tissues encounter the microbial environment of the gut. Their selective barrier and communication functions make them important for studying how microbes relate to tissue responses without treating the gut lining as a passive surface. This research connects epithelial biology with barrier integrity, inflammation, and disease mechanisms.