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Determination of Tolerable Fatty Acids and Cholera Toxin Concentrations Using Human Intestinal Epithelial Cells and BALB/c Mouse Macrophages
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Immunology and Infection
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JoVE Journal Immunology and Infection
Determination of Tolerable Fatty Acids and Cholera Toxin Concentrations Using Human Intestinal Epithelial Cells and BALB/c Mouse Macrophages

Determination of Tolerable Fatty Acids and Cholera Toxin Concentrations Using Human Intestinal Epithelial Cells and BALB/c Mouse Macrophages

DOI:
10.3791/50491-v

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09:39 min

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May 30, 2013

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, ,
1Department of Biological Sciences,Kingsborough Community College, 2Institute for Cellular and Molecular Biology,University of Texas at Austin, 3New Jersey Center for Science, Technology and Mathematics,Kean University

Chapters

  • 00:05Title
  • 00:59Tissue Culture
  • 02:24Fatty Acid Treatment
  • 04:12Cholera Toxin Treatment
  • 05:38MTT Assay
  • 06:47Results: Effects of Fatty Acids and Cholera Toxin on Cell Survival
  • 09:16Conclusion

Summary

Automatic Translation

Automatic Translation

We set out to determine tolerable concentrations of three fatty acids (oleic, linoleic and linolenic acids) and cholera toxin that did not significantly and adversely affect cell survival by solubilizing the fatty acids and the toxin and using them in cell survival assays.

Tags

Fatty AcidsCholera ToxinHuman Intestinal Epithelial CellsBALB/c Mouse MacrophagesCell Proliferation AssaysMucosal ImmunityCholera Prevention

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