Intestinal Glands
Intestinal Glands - Related Videos
04:37
Intestinal Crypts Isolation: A Method to Isolate Whole Crypts from Small Intestine of Murine Model
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2023
In this video, we demonstrate a technique to isolate whole crypts from a mouse’s small intestine. The isolated whole crypts can be used to propagate long-lived and self-renewing 3D organoids in vitro.
10:58
A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids
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Cited by 81 •
2015
In this video protocol we give a step by step explanation of lentiviral transduction in organoids of primary intestinal epithelium and of processing and downstream analysis of these cultures by quantitative RT-PCR, RNA-microarray and immunohistochemistry.
05:13
Gastric Fundic Glands Isolation: A Method to Isolate Fundic Glands from Human Stomach Tissue
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2023
This video describes a method to isolate gastric epithelial cells from the human fundic tissue. The isolated epithelial cells can be used as an in vitro model to study their regulatory roles in gastric physiology and pathophysiology.
11:49
Methods to Examine the Lymph Gland and Hemocytes in Drosophila Larvae
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Cited by 5 •
2016
Drosophila and mammalian hematopoietic systems share many common features, making Drosophila an attractive genetic model to study hematopoiesis. Here we demonstrate dissection and mounting of the major larval hematopoietic organ for immunohistochemistry. We also describe methods to assay various larval hematopoietic compartments including circulating hemocytes and sessile crystal cells.
04:20
Larval Lymph Gland Dissection: Isolating the Drosophila Hemocyte-Producing Organ
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2023
This video describes the Drosophila lymph gland—the primary site of hematopoiesis in the fly larva—and shows an example protocol to dissect and mount the organ for immunohistochemistry and fluorescent imaging.
FAQs
Stem cells continuously generate both absorptive and secretory epithelial cell types within the intestinal lining. This production system replaces cells that are lost during normal tissue activity and helps preserve the surface required for digestion and barrier function. Studying this lineage organization reveals how intestinal tissues balance renewal with specialized cellular roles.
These specialized cells contribute different secretory functions. Paneth cells release antimicrobial factors that help control microbes, goblet cells produce mucus that supports the epithelial barrier, and enteroendocrine cells release signaling hormones in response to local conditions. Their coordinated activity links gland function with microbial control, surface protection, nutrient processing, and intestinal communication.
Continuous epithelial renewal helps maintain the intestinal surface while digestion and local conditions place ongoing demands on the tissue. When researchers examine how renewal and specialized secretions are organized, they can better investigate epithelial homeostasis, inflammation, and inflammatory bowel disorders. The glands therefore provide a cellular framework for connecting repair processes with disease-related changes.
Intestinal glands inform organoid models designed to study epithelial organization and renewal outside the intestine. These models can represent relationships among stem cells and differentiated absorptive or secretory cells, allowing investigation of development, homeostasis, and responses relevant to intestinal biology. Their use also supports research aimed at understanding repair and evaluating concepts related to regenerative therapies.
Research on these glands can address how the intestinal lining develops, how epithelial cells are continuously replenished, and how secretory cell types contribute to digestion and barrier function. It can also examine microbial control and inflammation within the intestinal environment. Together, these questions connect cellular organization with normal gut maintenance and disorders involving epithelial dysfunction.
Because the glands contain stem cells that generate multiple epithelial cell types, they offer a system for studying how intestinal tissue is maintained and repaired. Findings from gland biology can guide organoid research and broader investigations of regenerative therapies. This context is especially relevant when researchers seek to understand or restore epithelial homeostasis in intestinal disease.