Intracellular Cytokine Staining
Intracellular Cytokine Staining - Related Videos
04:08
Analyzing the Effect of Tobacco Product Preparations on Cytokine Production via Intracellular Staining and Flow Cytometry
0 Views •
2025
This video demonstrates the effect of tobacco product preparations, TPPs, on cytokine production from human peripheral blood mononuclear cells using flow cytometry. Treatment of the cells with TPP downregulates the production of pro-inflammatory cytokines, which is estimated after lipopolysaccharide stimulation, followed by immunostaining and flow cytometry analysis.
03:40
Evaluation of Stromal Cytokine-Induced Intracellular Protein Phosphorylation Using Phospho-Flow Cytometry
0 Views •
2025
This video demonstrates the evaluation of stromal cytokine-induced intracellular phosphorylation of proteins in leukemia cells. These intracellular phosphorylated proteins are quantified using the phospho-flow cytometry technique.
06:57
In Vitro Culture for H5N1-Specific Duck T Cells and Detection of Immune Responses Using Intracellular Cytokine Staining Method
0 Views •
2025
The protocol describes a method for culturing avian T cells in vitro by isolating memory T cells from infected ducks, allowing for the generation of highly purified specific duck T cells. Additionally, an intracellular cytokine staining (ICS) method was established to accurately measure IFN-γ secretion in duck T cells.
09:20
Intracellular Staining and Flow Cytometry to Identify Lymphocyte Subsets within Murine Aorta, Kidney and Lymph Nodes in a Model of Hypertension
0 Views •
Cited by 8 •
2017
This article provides detailed methodology to identify and quantify functional T lymphocyte subsets present within murine kidney, aorta and lymph nodes by intracellular staining and flow cytometry. The model of angiotensin II induced hypertension was chosen to explain, step-by-step, the procedures and fundamental principles of flow cytometry and intracellular staining.
12:58
Expression of Exogenous Cytokine in Patient-derived Xenografts via Injection with a Cytokine-transduced Stromal Cell Line
0 Views •
Cited by 2 •
2017
Described here is a method for producing exogenous cytokine in patient-derived xenograft (PDX) mice via weekly intraperitoneal injection of a cytokine-transduced stromal cell line. This method broadens the utility of PDX and provides the option for transient or sustained exogenous cytokine delivery in a multitude of PDX models.
FAQs
Stimulation does more than initiate the assay: it promotes cytokine production so cells that can respond become detectable during the analysis. The experimental comparison therefore centers on the cytokine response elicited under the chosen stimulation condition. This makes intracellular cytokine staining useful for functional profiling rather than simply measuring which immune cells are present.
Blocking secretion causes newly produced cytokines to remain inside cells long enough for detection. Without intracellular retention, cytokine proteins would not be available to the antibody-based measurement described by this technique. This step links cellular function to a measurable fluorescence signal, allowing flow cytometry to identify cytokine-producing cells rather than only cells defined by their surface phenotype.
These preparation steps make antibody access possible inside the cell. Permeabilization allows fluorescently labeled antibodies to enter, while the cells are fixed before the intracellular targets are assessed. Together, they enable detection of cytokines retained within cells and support fluorescence measurements for individual cells during flow cytometry.
Surface markers add cellular identity to cytokine detection. By measuring these markers together with intracellular fluorescence, flow cytometry can distinguish which immune cell populations contain a given cytokine. This combination is important because the result describes both function, represented by cytokine production, and population membership, represented by the measured surface-marker pattern.
The method can quantify the proportion of cells producing a particular cytokine within analyzed immune populations. Because measurements occur at single-cell resolution, researchers can assess response patterns across defined populations rather than receiving only a combined signal from the entire sample. This makes the output suitable for comparing cellular immune responses.
Intracellular cytokine staining is especially relevant when a study asks which immune cells respond functionally to vaccination, infection, or an immune-modulating treatment. It can support functional profiling of lymphocytes and broader analysis of cellular immune responses. In these settings, pairing cytokine detection with surface markers helps connect treatment or exposure to specific responding populations.