Intradermal Injection
Intradermal Injection - Related Videos
08:46
Myeloid Cell Isolation from Mouse Skin and Draining Lymph Node Following Intradermal Immunization with Live Attenuated Plasmodium Sporozoites
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Cited by 12 •
2016
We describe here a protocol for isolating myeloid cells from mouse skin and draining lymph node following intradermal injection of Plasmodium sporozoites. Flow cytometry of collected cells provides a reliable assay to characterize the skin and draining lymph node inflammatory response to the parasite.
02:19
Intradermal Immunization with Live Attenuated Sporozoites in a Mouse Model
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2025
This video demonstrates an assay for immunizing a mouse model with live attenuated sporozoites. The radiation-attenuated Plasmodium sporozoites are injected into the dorsal side of the mouse's ear. After injections at multiple locations on both ears, the mouse is allowed to recover.
02:43
Tumor Implantation and Injection of a Tumor Vaccine in a Mouse Model
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2025
This video demonstrates a cell-based tumor vaccination assay. A mouse, intradermally injected with B16-F10 melanoma cells for tumor formation, is vaccinated with B16-F10 cells secreting Flt3L cytokine near the developed tumor. The secreted Flt3L from the vaccine promotes the maturation and proliferation of dendritic cells that then present tumor antigens to T cells in lymph nodes, triggering a targeted anti-tumor immune response.
09:20
Intradermal Inoculation of Mycobacterium avium in the Mouse Ear
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2025
Here we present a protocol for performing a low-dose intradermal inoculation of Mycobacterium avium in a mouse ear to reflect exposure-like conditions.
08:21
Intradermal Microdialysis: An Approach to Investigating Novel Mechanisms of Microvascular Dysfunction in Humans
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Cited by 2 •
2023
Intradermal microdialysis is a minimally invasive technique used to investigate microvascular function in health and disease. Both dose-response and local heating protocols can be utilized for this technique to explore mechanisms of vasodilation and vasoconstriction in the cutaneous circulation.
FAQs
The injected fluid remains near dermal immune cells and sensory structures instead of quickly moving into deeper tissue. This positioning allows the delivered material to contact local biological components within a confined area. In biology research, that local interaction is useful for examining how skin-associated immune activity or sensory responses develop around a defined deposit.
A confined deposit makes the resulting reaction easier to associate with the delivered material and the surrounding dermal environment. Rather than dispersing rapidly, the fluid remains available for local interaction, supporting observation of antigen sensitivity and immune activity. This localized behavior also helps researchers study delivery strategies designed specifically for skin-based responses.
The key distinction is where the material is deposited and how widely it can disperse. Intradermal injection places a small volume within the dermis, whereas deeper delivery moves material beyond that skin layer. Consequently, the intradermal approach emphasizes localized contact with dermal immune cells and sensory structures rather than rapid distribution through deeper tissue.
A localized reaction can provide evidence of antigen sensitivity or immune activity at the injection site. Because the response remains confined and observable, researchers can assess how the skin reacts to the delivered material without relying only on effects distributed throughout deeper tissue. This makes the technique relevant to investigations of local immune responses in biology and biomedical research.
The procedure centers on placing a small volume into the dermis with a needle entering the skin at a shallow angle. After the localized deposit is made, researchers observe the resulting site-specific reaction and assess the relevant biological response. The essential procedural goal is accurate placement within the dermis rather than deeper tissue delivery.
Researchers may select this technique for tuberculosis testing, allergy testing, selected vaccinations, or experiments focused on local immune responses. Its value comes from combining dermal placement with a confined, observable reaction. It can therefore help assess antigen sensitivity, evaluate immune activity near the skin, and investigate strategies for delivering materials through or into skin tissue.