Myeloid Cell Isolation from Mouse Skin and Draining Lymph Node Following Intradermal Immunization with Live Attenuated Plasmodium Sporozoites

The overall goal of this set of procedures is to immunize mice with radiation attenuated plasmodium parasites. To facilitate the analysis of myeloid cell recruitment in the mouse skin and draining lymph nodes. This method can help answer key questions in the malaria field, such as what are the cellular actors involved in the innate immunoresponse to live attenuated parasites.

Between 18-25 days after the infections blood meal, collect and cold anesthetize infected mosquitoes in a 15 milliliter tube on ice. Next, carefully invert the tube into a Petri dish on ice and expose the insects to a 12 kilorad dose of gamma or X-irradiation. Collect the irradiated mosquito salivary glands in 15 microliters of DPBS on ice.