Inverted Microscopy
Inverted Microscopy - Related Videos
06:13
The Inverted-face Effect
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2023
Source: Laboratory of Jonathan Flombaum—Johns Hopkins University In perception, it is often the case that the ability to recognize and interpret complex stimuli feels effortless but actually demands complicated and intensive processing. This is because processing is specialized and automated for certain types of very important stimuli. Among the best examples of this phenomenon is face processing. People do not try to detect and recognize faces. It just seems to happen. However, detecting faces...
01:20
Inverting and Non-inverting OpAmps
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2024
In an inverting amplifier, the input voltage is connected through a resistor to the inverting terminal. Meanwhile, the non-inverting terminal is grounded and a feedback resistor is established between the inverting and output terminal, as depicted in Figure 1. Figure 1: The inverting amplifier The objective is to discern the relationship between the input voltage (vi) and the output voltage (vo). By applying Kirchhoff's Current Law (KCL) and presuming the operational amplifier to be ideal, the...
09:51
Single Phase Inverter
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2023
Source: Ali Bazzi, Department of Electrical Engineering, University of Connecticut, Storrs, CT. DC power is unidirectional and flows in one direction, whereas, AC current alternates directions at a frequency of 50-60 Hz. Most common electronic devices are designed to run off of AC power; therefore an input DC source must be inverted to AC. Inverters convert DC voltage to AC through switching action that repeatedly flips the polarity of the input DC source at the output or load side for part of...
03:39
Inverted Motility Assay: An In Vitro Technique to Visualize Myosin Movement on Immobilized Actin Filaments
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2025
In this video, we perform an in vitro motility assay to analyze the actin-myosin interactions. The gliding movement of actin filaments on immobilized myosin molecules is visualized using fluorescence microscopy.
07:59
The Inverted Heart Model for Interstitial Transudate Collection from the Isolated Rat Heart
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2017
This protocol describes a method to collect cardiac interstitial fluid from the isolated, perfused rat heart. To physically separate interstitial transudate from coronary venous effluent perfusate, the Langendorff perfused heart is inverted, and the transudate (interstitial fluid) formed on the cardiac surface is collected using a soft latex cap.
FAQs
The below-sample placement lets the objective reach the imaging plane through the base of a culture dish or multiwell plate. It also keeps the upper side of the vessel available for handling, so imaging can be combined with media exchange, manipulation, or direct experimental monitoring.
Phase contrast is useful when cells are transparent and would be difficult to distinguish by ordinary transmitted-light viewing. It increases the visibility of cellular features without requiring a stain, allowing living samples to be observed in culture. This is particularly valuable when repeated observations are needed during growth, migration, or division.
These vessels hold specimens above the objective, allowing the optical system to view samples from below while the experiment remains contained in its dish or well. Their format is useful for biological work involving cultured cells, embryos, or tissues, and it supports observation across multiple wells when that format is used.
Researchers place the biological sample in a suitable dish, well, or other vessel, position the vessel over the objective, and select an appropriate transmitted-light contrast method. They can then observe the sample directly and return to it over time. The workflow also leaves access for media exchange or experimental manipulation during monitoring.
It is well suited to adherent cells and cell cultures that remain attached to a vessel surface, as well as embryos and tissues held in vessels. Imaging can follow growth, migration, and cell division, so the method supports both inspection of biological samples and time-lapse studies of changing behavior.
Sequential images can show how a sample changes rather than providing only a single visual snapshot. In the biological examples supported here, researchers can monitor growth, migration, and division over time. This makes the approach useful for relating visible changes in cells, embryos, or tissues to the progress of an experiment.