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The Inverted Heart Model for Interstitial Transudate Collection from the Isolated Rat Heart
 

The Inverted Heart Model for Interstitial Transudate Collection from the Isolated Rat Heart

Article DOI: 10.3791/55849
June 20th, 2017

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Summary June 20th, 2017

This protocol describes a method to collect cardiac interstitial fluid from the isolated, perfused rat heart. To physically separate interstitial transudate from coronary venous effluent perfusate, the Langendorff perfused heart is inverted, and the transudate (interstitial fluid) formed on the cardiac surface is collected using a soft latex cap.

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