Microarray Printing
Microarray Printing - Related Videos
13:37
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Cited by 33 •
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A description of the formation of a polymer microarray using an on-chip photopolymerization technique. The high throughput surface characterization using atomic force microscopy, water contact angle measurements, X-ray photoelectron spectroscopy and time of flight secondary ion mass spectrometry and a cell attachment assay is also described.
07:04
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A simple procedure of performing custom microRNA microarray experiments is described. The steps include isolating RNA, labeling RNA and reference DNA, hybridizing the samples to microarrays, scanning the microarrays, quantifying and analyzing hybridization signals.
11:57
Fabrication and Use of MicroEnvironment microArrays (MEArrays)
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Cited by 25 •
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09:47
Analysis of Histone Antibody Specificity with Peptide Microarrays
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Cited by 22 •
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This manuscript describes methods for applying peptide microarray technology to specificity profiling of antibodies that recognize histones and their post-translational modifications.
05:21
A Peptide Microarray Technology for Specificity Profiling of Antibodies
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2025
This video demonstrates a technique that applies peptide microarray technology to profile the specificity of antibodies recognizing post-translational modifications (PTMs) on histone peptides. A microarray slide containing immobilized fluorophore-conjugated histone peptides with known PTM combinations is treated with target PTM-specific antibodies. This process helps identify the antibody's specificity for the target and its potential cross-reactivity with non-target PTMs.
FAQs
Contact pins transfer tiny volumes by physically touching the solid surface, whereas inkjet dispensers deposit probe solution without the same pin-contact step. Both approaches create defined, addressable spots, but they represent different delivery mechanisms for arranging DNA, RNA, or protein probes. The selected system determines how probe solutions are placed into the patterned array.
Drying helps retain each small deposit as a discrete spot, while chemical binding can attach probes to the substrate. These steps preserve the array’s spatial organization so each location remains associated with a known probe. Maintaining that addressability is essential when later binding or hybridization signals are assigned to particular biological targets.
After labeled biological samples contact the printed surface, molecules can hybridize or bind to matching probes. The labels make those interactions detectable as signals at specific array locations. Researchers can then measure and compare the signals across spots, using the spatial pattern to determine which probes interacted with sample molecules and to assess relative biological differences.
A typical workflow places selected probe solutions into a robotic contact-pin or inkjet dispensing system, deposits them in a defined pattern on a solid substrate, and allows the spots to dry or bind chemically. The completed array is then exposed to labeled samples, followed by detection and comparison of signals produced at matching probe locations.
The method can arrange DNA, RNA, or protein probes, allowing the same general platform to address different biological measurements. Supported uses include gene expression profiling, genotyping, biomarker studies, and multiplex assays. The probe type and sample interaction determine which biological targets are examined and what pattern of measured signals is interpreted.
Microarray printing places many probes in parallel, so a single patterned surface can support analysis of numerous biological targets. This organization enables multiplex measurements while conserving sample and reagent volumes. In biology, that combination is useful for profiling gene expression, examining genotypes, investigating biomarkers, and comparing multiple binding or hybridization outcomes in one assay.