Analysis of Histone Antibody Specificity with Peptide Microarrays

The goal of this video is to describe an integrated pipeline for the design fabrication and use of peptide microarrays for profiling the specificities of histone antibodies. Antibodies that recognize histones and their diverse post-translational modifications are key reagents in chromatin biochemistry research. We rely on these tools to deepen our understanding of histone modification function.

Recent studies reveal inconsistencies in histone antibody behavior. As antibody choice is the most critical element in epigenetics experiments, vigorous incomprehensive quality control measures are demanded for these reagents. Here, we demonstrate an integrated pipeline we'd developed for designing, fabricating and analyzing peptide microarrays to generate specificity profiles for antibodies that recognize histones and their post-translational modifications.