Microscope Software

Microscope software is a set of digital tools that controls image acquisition, processes microscope data, and helps researchers interpret biological specimens. It communicates with microscope hardware and cameras to adjust parameters such as focus, illumination, exposure, and magnification, then applies operations such as image enhancement, segmentation, tracking, and measurement to recorded images. In biology, these functions support the visualization and quantitative analysis of cells, tissues, microorganisms, and dynamic events such as cell movement or growth. By improving reproducibility and reducing manual analysis, microscope software enables high-throughput imaging, time-lapse studies, and more consistent comparisons across experiments.

Microscope Software - Related Videos

Research

JoVE Journal - Biology
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Imaging C. elegans Embryos using an Epifluorescent Microscope and Open Source Software

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Cited by 8 •

2011

The C. elegans embryo is a powerful system for studying cell biology and development. We present a protocol for live imaging of C. elegans embryos utilizing DIC optics or fluorescence using readily available epifluorescent microscopes and open-source software.

Research

JoVE Journal - Biology
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Workflow for High-content, Individual Cell Quantification of Fluorescent Markers from Universal Microscope Data, Supported by Open Source Software

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Cited by 5 •

2014

Presented is a flexible informatics workflow enabling multiplexed image-based analysis of fluorescently labeled cells. The workflow quantifies nuclear and cytoplasmic markers and computes marker translocation between these compartments. Procedures are provided for perturbation of cells using siRNA and reliable methodology for marker detection by indirect immunofluorescence in 96-well formats.

Research

JoVE Journal - Neuroscience

VisualEyes: A Modular Software System for Oculomotor Experimentation

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Cited by 28 •

2011

Neural control and cognitive processes can be studied through eye movements. The VisualEyes software allows an operator to program stimuli on two computer screens independently using a simple, custom scripting language. The system can stimulate tandem eye movements (saccades and smooth pursuit) or opposing eye movements (vergence) or any combination.

Research

JoVE Journal - Neuroscience
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Automated Analysis of C. elegans Swim Behavior Using CeleST Software

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Cited by 18 •

2016

An efficient and simple methodology for computer-based analysis of nematode swimming behavior in liquid is described. The method requires little to no investment for C. elegans laboratories. The hardware used is standard, and the computer software for the behavioral analysis (CeleST) is an open source one.

Research

JoVE Journal - Biology
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Proper Care and Cleaning of the Microscope

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Cited by 2 •

2008

Keeping the microscope optics clean is important for high-quality imaging. Dust, fingerprints, excess immersion oil, or mounting medium on or in a microscope causes reduction in contrast and resolution. DIC is especially sensitive to contamination and scratches on the lens surfaces. This protocol details the procedure for keeping the microscope clean.

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