Mutant Bacteria
Mutant Bacteria - Related Videos
05:13
Assessment of Intracellular Growth of Coxiella Mutants within Eukaryotic Cells
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2025
This video demonstrates a method for assessing the intracellular growth of GFP-tagged Coxiella mutants within cultured epithelial cells. The mutant phenotype is visualized using immunostaining and epifluorescence microscopy, allowing the study of the effect of the mutation on intracellular growth.
11:44
Generation and Multi-phenotypic High-content Screening of Coxiella burnetii Transposon Mutants
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Cited by 28 •
2015
Coxiella burnetii is an obligate intracellular Gram-negative bacterium responsible for the zoonotic disease Q fever. Here we describe methods for the generation of Coxiella fluorescent transposon mutants as well as the automated identification and analysis of the resulting internalization, replication and cytotoxic phenotypes.
09:17
Generation of Maternal Mutants Using zpc:cas9 Knock-in Zebrafish
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2025
Here, we describe a protocol for generating maternal mutant that couples a stable zpc:cas9 knock-in line with Tol2-mediated delivery of sgRNA expression cassettes.
18:01
Paradigms for Pharmacological Characterization of C. elegans Synaptic Transmission Mutants
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Cited by 20 •
2008
This video demonstrates how to employ two neural stimulants, aldicarb and pentylenetetrazole (PTZ), in complementary ways to study synaptic function in the nematode, C. elegans. This complementary approach may also be used to shed light on evolutionarily conserved mechanisms for modulating neuronal synchrony and has implications for epilepsy and seizures.
02:52
Expansion of Mutant Vaccinia Virus in Mammalian Cells
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2026
Source: Yuan, M., et al. A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors. J. Vis. Exp. (2016)This video demonstrates the expansion of plaque-derived recombinant virus in adherent mammalian cells. The virus carries a fluorescent reporter in place of a virulent gene, enabling infection tracking. Infected cells are harvested for PCR verification of the genetic modification.
FAQs
Replication errors and DNA damage can change bacterial DNA, but their effects become biologically informative when the altered sequence is inherited by subsequent cells. Laboratory mutagens provide a controlled way to increase the chance of obtaining variants, while comparison with a reference strain helps distinguish mutation-associated traits from ordinary variation. This supports gene-function studies in biology.
Selection favors bacterial variants that survive a particular condition, so the environment determines which traits become more represented. For example, exposure to an antibiotic can favor cells with altered antibiotic susceptibility, while another condition may favor changes affecting metabolism or cell structure. Studying this process helps explain adaptation and the emergence of antimicrobial resistance.
Researchers can examine changes in metabolism, cell structure, virulence, and antibiotic susceptibility to connect altered traits with affected genes. A mutant strain and its reference strain provide a useful comparison because differences between them can reveal how particular genetic changes influence cellular function. This approach turns variation among bacterial cells into evidence about gene roles.
A basic investigation can begin by obtaining variants through naturally arising changes or laboratory mutagens, then comparing those variants with a reference strain. Researchers expose the strains to relevant conditions and examine differences in traits such as metabolism, cell structure, virulence, or antibiotic susceptibility. The resulting comparisons help associate inherited changes with observable biological outcomes.
Strains with altered antibiotic susceptibility allow researchers to examine how bacterial genetic changes affect survival during antimicrobial exposure. Selection can favor variants that persist under those conditions, providing a model for studying resistance emergence. These findings are relevant to infection-control strategies because they clarify how environmental pressure can shape bacterial populations.
Mutant strains help researchers investigate adaptation, cellular function, and responses to environmental stress. They also support work on diagnostic tools, engineered microbes, and infection control by revealing how genetic changes influence bacterial traits. In biology, these applications connect controlled studies of bacterial variation with practical efforts to understand and manage microbial behavior.