Murine Skin Tumors
Murine Skin Tumors - Related Videos
07:59
Murine Full-thickness Skin Transplantation
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Cited by 41 •
2017
Murine full-thickness skin transplantation is a well-established model to study rejection in an alloimmune setting. Here, we provide a tutorial of each step involved in performing a BALB/c-->C57BL/6 full-thickness skin transplant.
06:42
Murine Skin Transplantation
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Cited by 36 •
2008
Allogeneic skin transplantation is a standard model to assay host T cell responses to MHC-disparate donor antigens. This video-article provides a visual tutorial of each step involved in performing a BALB/c-->C57BL/6 skin transplant.
08:39
Quantifying Leukocyte Egress via Lymphatic Vessels from Murine Skin and Tumors
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Cited by 23 •
2019
Here, we demonstrate the methods for in vivo quantification of leukocyte egress from naïve, inflamed, and malignant murine skin. We perform a head-to-head comparison of two models: transdermal FITC application and in situ photoconversion. Furthermore, we demonstrate the utility of photoconversion for tracking leukocyte egress from cutaneous tumors.
04:23
Frozen Skin Tissue Block Preparation: A Protocol to Preserve Cutaneous Melanoma Samples from Murine Dorsal Skin
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2023
This video describes the detailed protocol for isolating melanoma-containing murine dorsal skin, followed by cryo-embedding to prepare a frozen skin tissue block. The cryo-embedded tissue block can be sectioned and microscopically examined to study melanoma progression.
06:23
A Murine Orthotopic Bladder Tumor Model and Tumor Detection System
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Cited by 8 •
2017
This protocol describes the generation of murine orthotopic bladder tumors in female C57BL/6J mice and the monitoring of tumor growth.
FAQs
Genetic changes can alter the controls that regulate cell growth, while epigenetic changes can modify how cellular instructions are used without changing the underlying sequence. In murine skin tumor studies, these disruptions are examined through their effects on differentiation, continued expansion, and communication with surrounding tissue. Linking the initiating alteration to later tumor behavior helps clarify how carcinogenesis unfolds over time.
These stages represent different aspects of tumor development. Initiation focuses on the events that establish abnormal growth, progression addresses how the lesion develops over time, and invasion concerns movement into surrounding tissue. Evaluating them separately helps researchers determine whether a model reproduces only early carcinogenesis or also captures later biological changes relevant to cancer behavior.
The surrounding microenvironment provides a biological context for examining how abnormal cells interact with nearby tissue. These interactions are considered alongside changes in growth control and differentiation, rather than treating the tumor as an isolated group of cells. Studying the tissue response in a living mouse can therefore connect tumor behavior with broader local effects during carcinogenesis.
These approaches reproduce tumor development through different experimental starting points: exposure-based induction, engineered genetic conditions, or transplantation. Comparing them allows investigators to examine whether observed features depend on how the tumor model was established or appear across multiple systems. This range supports broader analysis of carcinogenesis than reliance on a single experimental route.
These models can reveal how tumors begin, develop, invade surrounding tissue, and respond to treatment in a living organism. Such observations help investigators connect an intervention with changes in tumor behavior rather than assessing only isolated cellular effects. The resulting evidence can support preclinical evaluation of both cancer-prevention approaches and therapeutic strategies.
Their value comes from combining controlled experimental models with observation of cancer-related processes in living tissue. Researchers can investigate tissue responses and tumor development while testing how findings may relate to human skin cancer. This translational context makes the models useful for studying mechanisms and for generating preclinical evidence before preventive or therapeutic strategies are advanced.