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1Department of Cell, Developmental, & Cancer Biology,Oregon Health and Science University, 2Department of Molecular Microbiology & Immunology,Oregon Health and Science University, 3Department of Dermatology,Oregon Health and Science University, 4Knight Cancer Institute,Oregon Health and Science University
Chapters
- 00:04Title
- 00:46Mouse Pinna Inflammation Induction, Vaccinia Infection, Photoconversion, and Tissue Processing
- 02:52Intradermal Melanoma Tumor Photoconversion and Tissue Processing
- 04:00Flow Cytometry Preparation & Analysis
- 05:18Results: Representative Photoconversion Efficiency in Skin and the Tumor Microenvironments
- 07:55Conclusion
Here, we demonstrate the methods for in vivo quantification of leukocyte egress from naïve, inflamed, and malignant murine skin. We perform a head-to-head comparison of two models: transdermal FITC application and in situ photoconversion. Furthermore, we demonstrate the utility of photoconversion for tracking leukocyte egress from cutaneous tumors.
