Needle Tip Bubbles
Needle Tip Bubbles - Related Videos
01:13
Excess Pressure Inside a Drop and a Bubble
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2023
The shape of a small drop of liquid can be considered spherical, neglecting the effect of gravity. This drop can further be considered as two equal hemispherical drops put together due to surface tension. The forces acting on the spherical drop are due to the pressure of the liquid inside the drop, the pressure due to air outside the drop, and the force due to the surface tension acting on the two hemispherical drops. where γ corresponds to the surface tension. Recalling that the force per...
09:51
Intraosseous Needle Placement
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2023
Source: Julianna Jung, MD, FACEP, Associate Professor of Emergency Medicine, The Johns Hopkins University School of Medicine, Maryland, USA For unstable patients requiring urgent administration of medications, fluids, or blood products, establishing vascular access quickly is essential. However, there are many factors that can complicate placement of a peripheral intravenous cannula (PIV), and it is extremely common for PIV attempts to fail. PIV placement may be technically challenging in small...
10:06
Personalized Needles for Microinjections in the Rodent Brain
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Cited by 3 •
2018
We describe here a protocol for microinjection in the rodent brain that uses quartz needles. These needles do not produce detectable tissue damage and ensure reliable delivery even in deep regions. Moreover, they can be adapted to research needs by personalized designs and can be re-used.
08:25
Needle Thoracostomy
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2015
Source: Rachel Liu, BAO, MBBCh, Emergency Medicine, Yale School of Medicine, New Haven, Connecticut, USA A tension pneumothorax is a life-threatening situation in which excess air is introduced into the pleural space surrounding the lung, either through trauma to the chest cavity or as a spontaneous leak of air from the lung itself. Air trapped within the pleural space causes separation of the lung from the chest wall, disrupting normal breathing mechanisms. Pneumothorax may be small without...
08:19
Induction of Microstreaming by Nonspherical Bubble Oscillations in an Acoustic Levitation System
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Cited by 2 •
2021
A fast and reliable technique is proposed to control the shape oscillations of a single, trapped acoustic bubble that is based on coalescence technique between two bubbles. The steady-state, symmetry-controlled bubble shape oscillations allow analysis of the fluid flow generated in the vicinity of the bubble interface.
FAQs
A gas pocket responds differently from the surrounding liquid when pressure is applied. Instead of transferring the intended liquid volume immediately, part of the applied pressure can compress the gas or allow it to expand. This decouples pressure from liquid movement, making the delivered amount less predictable during precise biological injections.
Pressure may initially change the size of the trapped gas pocket rather than move solution through the needle opening. Liquid release can therefore be delayed until the bubble’s response changes, followed by an uneven discharge. These fluctuations interfere with the steady, controlled delivery needed when very small volumes are introduced into biological targets.
Cells and embryos are injection targets that require controlled delivery of DNA, RNA, proteins, or other solutions. If a bubble causes the injection pressure to vary, the procedure may deliver solution irregularly and increase mechanical stress on the target. Preventing this source of instability supports more consistent handling and reduces the likelihood of damage.
Recognition alerts the experimenter to a potential source of inaccurate fluid delivery before it affects the biological sample. Because the bubble can absorb or release pressure without producing the expected liquid movement, identifying and preventing it helps maintain more predictable injection behavior. This is particularly relevant when reproducibility and gentle treatment of cells or embryos matter.
The problem is relevant wherever microinjection must deliver a defined solution into a cell or embryo. The overview specifically identifies DNA, RNA, proteins, and other solutions as materials that may be introduced. In each case, unstable release or fluctuating pressure can compromise delivery control and make comparisons among biological experiments less reliable.
Uncontrolled gas behavior can create irregular flow, delayed release, or unintended pressure fluctuations during injection. Those effects may alter how consistently the intended solution reaches cells or embryos and may increase mechanical stress on the target. Recognizing and preventing the problem therefore helps researchers obtain more reproducible biological experiments while reducing avoidable damage during microinjection.